Investigation Title	Transcription profiling of Arabidopsis HSI2 and HSI2-L1 double mutant 	
Comment[Submitted Name]	K.Nakamura-A.thaliana-HSI2 double mutant	
Experimental Design	genetic_modification_design	transcription profiling by array	
Experimental Design Term Source REF	mo	EFO	
Comment[ArrayExpressReleaseDate]	2007-02-02	
Comment[AEMIAMESCORE]	3	
Comment[ArrayExpressAccession]	E-MEXP-542	
Comment[MAGETAB TimeStamp_Version]	2010-08-11 14:46:05 Last Changed Rev: 13058	
Experimental Factor Name	genotype	
Experimental Factor Type	genotype	
Experimental Factor Term Source REF	
Person Last Name	Nakamura	
Person First Name	Kenzo	
Person Mid Initials	
Person Email	kenzo@agr.nagoya-u.ac.jp	
Person Phone	81-52-789-4095	
Person Fax	81-52-789-4094	
Person Address	Chikusa, Nagoya	
Person Affiliation	Graduate School of Bioagricultural Sciences	
Person Roles	submitter	
Person Roles Term Source REF		
Quality Control Type	
Quality Control Term Source REF	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2007-02-02	
PubMed ID	17267611	
Publication DOI	17267611	
Publication Author List	Hironaka Tsukagoshi, Atsushi Morikami, Kenzo Nakamura	
Publication Title	Two B3 domain transcriptional repressors prevent sugar-inducible expression of seed maturation genes in Arabidopsis seedlings	
Publication Status	journal_article	
Publication Status Term Source REF	
Experiment Description	The effect of HSI2 and HSI2-L1 double mutant on plant gene expression.	
Protocol Name	P-MEXP-16774	P-MEXP-16771	P-MEXP-16772	P-MEXP-16595	P-MEXP-16642	P-MEXP-16631	P-MEXP-16716	P-MEXP-16647	
Protocol Type	specified_biomaterial_action	grow	pool	nucleic_acid_extraction	labeling	labeling	hybridization	feature_extraction	
Protocol Description	4-day-old seedling plant  grown on the 1% sucrose MS medium	Arabidopsis thaliana seeds were sterilized in sterile water, kept 4C for 3-d, and sown on 0.3% (w/v) gellun gum plates containing Murashige and Skoog (MS) medium, 0.5 g/L MES, pH 5.8, 100 mg/L myoinositol, 10 mg/L thiamine-HCl, 1 mg/L nicotinic acid, 1 mg/L pyridoxine HCl, and 1% sucrose. Plates were incubated in growth chamber at 22C under continuous light (50 mmol photons m-2 s-1).	Approximately 10 individual plants were pooled.	Total RNA was extracted with RNeasy Plant Mini Kit(Qiagen) according to the manufacturer`s instructions.	Cy-5 labeled cRNA was synthesized using Low RNA Input Linear Amplification Kit (Agilent, product # 5184-3523) from total RNA and fragmented according to the manufacturer`s instructions.	Cy-3 labeled cRNA was synthesized using Low RNA Input Linear Amplification Kit (Agilent, product # 5184-3523) from total RNA and fragmented according to the manufacturer`s instructions.	Hybridization using In situ Hybridization Kit Plus (Agilent, product #5184-3568) according to the manufacturer's instructions (protocol: Part #G4140-96010)	PMT 100%	
Protocol Parameters		start time;stop time;min temperature;		Amplification;Extracted product;	Label used;Amount of nucleic acid labeled;Amplification;	Amount of nucleic acid labeled;Amplification;Label used;	time;temperature;Chamber type;Quantity of label target used;	
Protocol Hardware								G2565AA DNA microarray scanner [Agilent]	
Protocol Software								Feature Extraction Software [Agilent]	
Protocol Contact	
Protocol Term Source REF	mo	
SDRF File	E-MEXP-542.sdrf.txt	
Term Source Name	mo		The MGED Ontology	ncbitax	tair_dev:1.27	ArrayExpress	mo	EFO	
Term Source File	http://mged.sourceforge.net/ontologies/MGEDontology.php		http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ncbi.nlm.nih.gov/Taxonomy/taxonomyhome.html	http://www.arabidopsis.org/info/ontologies/	http://www.ebi.ac.uk/arrayexpress	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/efo/	
Term Source Version					1.27