Comment[ArrayExpressAccession] E-MEXP-2611 Investigation Title Transcription profiling by array of Bos taurus liver from control and either orally or intramuscularly DHEA treated animals. Comment[Submitted Name] Bovine DHEA exposure Publication Title Publication Author List Publication DOI PubMed ID Experimental Design co-expression_design compound_treatment_design in_vivo_design Experimental Design Term Source REF The MGED Ontology The MGED Ontology Comment[MIAMExpressLogin] Bovine Comment[MIAMExpressSubmissionID] 6506 Experiment Description Comparing gene expression profiles of bovines treated orally or intramuscularly with DHEA versus control animals. Experimental Factor Name COMPOUND DOSE ClinicalInformation Age Experimental Factor Type compound dose clinical_information age Person Last Name Rijk Person First Name Jeroen Person Email jeroen.rijk@wur.nl Person Phone 0031-317-480358 Person Affiliation RIKILT - Institute of Food Safety, Wageningen UR Person Address Toxicology and Effectmonitoring, Akkermaalsbos 2, Wageningen, --, 6708 WB, Netherlands Person Roles submitter Person Roles Term Source REF MGED Ontology Quality Control Type spike_quality_control Public Release Date 2011-03-09 Comment[ArrayExpressSubmissionDate] 2010-03-10 Protocol Name P-MTAB-12789 P-MTAB-12790 P-MTAB-12791 P-MTAB-12792 P-MTAB-12793 P-MTAB-12794 Protocol Description Total RNA was extracted from liver tissue by homogenation in Trizol.

RNA was purified using RNeasy mini kit protocol (Qiagen)

Fluorescently-labeled cRNA was generated using the Agilent Low RNA Input Fluorescent Linear Amplification Kit according tot the manufacturer's protocol.
(Parameters: Extracted product = total_RNA, Amplification = OTHER: Agilent Low RNA Input Fluorescent Linear Amplification Kit) Normalization performed as described by:

Pellis L, Franssen-van Hal NLW, Burema J, Keijer J, 2003. The interclass correlation coefficient applied for evaluation of data correction, labeling methods, and rectal biopsy sampling in DNA microarray experiments. Physiol. Genomics 16, 99-106. RNA of all control animals was pooled Male fresian bull calves were purchased from the local market and housed for 2-3 weeks before the start of the experiment.Treatment with DHEA was repeated in three independent experiments using identical treatment schedules. Each of the three experiments consisted of two animals of which one was orally (PO) treated and the other was intramuscular (IM) treated. Administrations were performed seven times at 24 hour intervals. IM treated animals (n=3) were 9-12 months old, PO treated animals (n=3)were 8-13 months old. Control animals were included in the first (n=3, all 6 months old) and in the third (n=4, 13-14 months old)experiment. The animals were housed under normal livestock conditions. At the end of the experiment the animals were sacrificed, liver tissue was collected and snap frozen in liquid nitrogen.
(Parameters: start time = 1, stop time = 7, time unit = days, temperature unit = C) Normalization performed as described by:

Pellis L, Franssen-van Hal NLW, Burema J, Keijer J, 2003. The interclass correlation coefficient applied for evaluation of data correction, labeling methods, and rectal biopsy sampling in DNA microarray experiments. Physiol. Genomics 16, 99-106. The values were further log2 transformed. DHEA exposed animals wer treated either orally (PO) with capsules containing 1000 mg DHEA or intramuscular (IM) with 1000 mg DHEA disolved in 10 mL Miglyol. Administrations were performed 7 times at 24h intervals. Protocol Type nucleic_acid_extraction bioassay_data_transformation pool grow bioassay_data_transformation specified_biomaterial_action Protocol Term Source REF MGED Ontology MGED Ontology MGED Ontology MGED Ontology MGED Ontology MGED Ontology Term Source Name MGED Ontology ArrayExpress The MGED Ontology EFO Term Source File http://mged.sourceforge.net/ontologies/MGEDontology.php http://www.ebi.ac.uk/arrayexpress http://efo.svn.sourceforge.net/viewvc/efo/trunk/src/efoinowl/efo.owl SDRF File E-MEXP-2611.sdrf.txt