Investigation Title	Transcription profiling of human and macacque liver and muscle tissues	
Comment[Submitted Name]	MB_UH1	
Experimental Design	species_design	transcription profiling by array	
Experimental Design Term Source REF	mo	EFO	
Comment[ArrayExpressReleaseDate]	2005-05-01	
Comment[AEMIAMESCORE]	4	
Comment[ArrayExpressAccession]	E-MEXP-216	
Comment[MAGETAB TimeStamp_Version]	2010-08-10 11:24:47 Last Changed Rev: 13058	
Experimental Factor Name	Species	Organism Part	
Experimental Factor Type	organism	organism_part	
Experimental Factor Term Source REF	
Person Last Name	Chalwatzis	Hemmann	Kotlenga	Lindauer	Von Buelow	
Person First Name	Nicolas	Ulrike	Katja	Klaus	Manon	
Person Mid Initials	
Person Email					Manon.vonBuelow@aventis.com	
Person Phone					+49 (0)69 305 46348	
Person Fax	
Person Address					Industriepark Hoechst, G879	
Person Affiliation	sanofi-aventis	sanofi-aventis	sanofi-aventis	sanofi-aventis	Functional Genomics	
Person Roles					submitter	
Person Roles Term Source REF						
Quality Control Type	technical_replicate	spike_quality_control	biological_replicate	
Quality Control Term Source REF	mo		The MGED Ontology	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2005-05-01	
PubMed ID	
Publication DOI	
Publication Author List	 Manon von Buelow; Ulrike Hemmann; Nicolas Chalwatzis; Katja Kotlenga; Klaus Lindauer	
Publication Title	Gene Expression Analysis of Non-human Primates: Use of a Probe Match Table to Improve the Interpretation of Cross-Species Hybridizations on Microarrays.	
Publication Status	
Publication Status Term Source REF	
Experiment Description	This experiment was performed to improve gene expression analysis of non-human primates using human microarrays. To improve the interpretation of cross-species hybridizations a probe match table was created. We validated this table by comparing gene expression data obtained from human and M. fascicularis. Two tissues were used in this comparison: Liver and muscle.	
Protocol Name	P-MEXP-5951	P-MEXP-5952	P-MEXP-5953	P-MEXP-5954	P-AFFY-6	
Protocol Type	specified_biomaterial_action	nucleic_acid_extraction	labeling	hybridization	feature_extraction	
Protocol Description	Macaca fascicularis have been treated with 0.5 % tylose H 4000 in purified water, 5 ml/kg/day, administered once daily for 42 days.	Macaca fascicularis samples: Muscle total RNA was extracted using Trizol Reagent (Invitrogen Life Technologies, Carlsbad, CA) as described by the manufacturer. Liver samples were homogenized in RLT buffer (Qiagen, Hilden, Germany) using a MixerMill MM300. RNA was purified on RNeasy Mini columns (Qiagen, Hilden, Germany) for RNA cleanup and DNase treatment (RNase-Free DNase Set Protocol, Qiagen, Hilden, Germany).  Human samples: Human total RNA was obtained from commercial providers.	RNA was converted into double-stranded cDNA by using the Superscript Double-Stranded cDNA Synthesis Kit (Invitrogen Life Technologies, Carlsbad, CA). Biotin-labelled cRNA synthesis was carried out using BioArray HighYield RNA Transcript Labeling Kit (Enzo Life Science, Farmingdale, NY). 10 ug of fragmented cRNA were loaded per array. 	Hybridization, washing, staining, and scanning of Affymetrix GeneChip HG-U133A oligonucleotide arrays (Affymetrix Inc, Santa Clara, CA) was carried out according to the Affymetrix technical manual (Lockhart et al., 1996) in an Affymetrix hybridization oven 640 and an Affymetrix fluidics station. The fluidics protocol used was EukGE-WS2v4.	Title: Affymetrix CEL analysis. Description: 	
Protocol Parameters		Amplification;Extracted product;	Label used;Amplification;Amount of nucleic acid labeled;	temperature;time;Volume;Chamber type;Quantity of label target used;	
Protocol Hardware	
Protocol Software					MicroArraySuite 5.0	
Protocol Contact	
Protocol Term Source REF		mo	mo	mo	
SDRF File	E-MEXP-216.sdrf.txt	
Term Source Name	mo	cbil_cv	NCI_thesaurus	ma		nci_meta	ArrayExpress	mo	The MGED Ontology	EFO	
Term Source File	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.cbil.upenn.edu/anatomy.php3	ncithesaurus.obo.alt	http://berkeleybop.org/cgi-bin/obofoundry/detail.cgi?id=adult_mouse_anatomy		http://ncimeta.nci.nih.gov/indexMetaphrase.html	http://www.ebi.ac.uk/arrayexpress	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/efo/	
Term Source Version