Investigation Title	Transcription profiling of mouse embyonic fibroblasts from wild type and Elavl1knock-out embryos	
Comment[Submitted Name]	MEFs HuR	
Experimental Design	ex_vivo_design	genetic_modification_design	co-expression_design	transcription profiling by array	
Experimental Design Term Source REF		The MGED Ontology	The MGED Ontology	EFO	
Comment[AEMIAMESCORE]	3	
Comment[SecondaryAccession]		
Comment[ArrayExpressReleaseDate]	2009-03-15	
Comment[ArrayExpressAccession]	E-MEXP-1785	
Comment[MAGETAB TimeStamp_Version]	2011-06-28 19:24:24 Last Changed Rev: 14857	
Experimental Factor Name	GENOTYPE	
Experimental Factor Type	genotype	
Experimental Factor Term Source REF	
Person Last Name	Harokopos	
Person First Name	Vaggelis	
Person Mid Initials	
Person Email	harokopos@fleming.gr	
Person Phone	-9656490	
Person Fax	-9654114	
Person Address	P.O. Box 74145, Varkiza, Attiki, 16602, Greece	
Person Affiliation	B.S.R.C. Al. Fleming	
Person Roles	submitter	
Person Roles Term Source REF		
Quality Control Type	biological_replicate	
Quality Control Term Source REF	The MGED Ontology	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2009-03-15	
PubMed ID	
Publication DOI	
Publication Author List	
Publication Title	The RNA-binding protein Elavl1/HuR is essential for placental branching morphogenesis and embryonic development	
Publication Status	
Publication Status Term Source REF	
Experiment Description	Mouse embryonic fibroblasts (MEF) were isolated via enzymatic dissociation of 3 E14.5 embrya bearing epiblast specific inactivation of the murine Elavl1 locus as 3 littermate controls. Individual Fibroblast cultures were maintained in complete DMEM medium containing 10%FBS at 370C with 5%CO2. Cultures were passaged for 3 times and harvested for RNA isolation when they reached 60-70% conflunecy.	
Protocol Name	P-MTAB-1606	P-MTAB-1605	P-AFFY-2	Affymetrix:Protocol:Hybridization-EukGE-WS2[]	P-AFFY-6	Affymetrix:Protocol:MAS4:ExpressionCall	
Protocol Type	grow	nucleic_acid_extraction	labeling	hybridization	feature_extraction	bioassay_data_transformation	
Protocol Description	Individual Fibroblasts cultures were maintained in complete DMEM medium containing 10%FBS at 37C with 5%CO2. Cultures were passaged for 3 times and harvested for RNA isolation when they reached 60%-70% confluency <br>(Parameters: start time = 3, time unit = days, min temperature = 37, temperature unit = C, media = DMEM)	Total RNA extracted with TRIzol reagent (Life Technologies, Rockville, Maryland, United States)according to the manufacturer’s instructions and purified with an RNeasy Mini Kit Qiagen (Hilden, Germany).<br>(Parameters: Extracted product = total_RNA, Amplification = none)	Title: Affymetrix in vitro transcription. Description: 	Title: Affymetrix EukGE-WS2 Hybridization. Description: 	Title: Affymetrix CEL analysis. Description: 	Title: Affymetrix CHP Analysis (ExpressionCall). Description: 	
Protocol Parameters	
Protocol Hardware	
Protocol Software				MicroArraySuite 5.0	MicroArraySuite 5.0	MicroArraySuite 4.0	
Protocol Contact	
Protocol Term Source REF				mo		mo	
SDRF File	E-MEXP-1785.sdrf.txt	
Term Source Name	The MGED Ontology		NCI_thesaurus	ArrayExpress	The MGED Ontology	EFO	mo	
Term Source File	http://mged.sourceforge.net/ontologies/MGEDontology.php		ncithesaurus.obo.alt	http://www.ebi.ac.uk/arrayexpress	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	
Term Source Version