Investigation Title Transcription profiling by array of mouse microvascular endothelial cells treated with FGF2 Comment[AdditionalFile:XLS] E-MEXP-1467.hybs.xls Comment[Submitted Name] A Pro-inflammatory Signature Mediates FGF2-induced Angiogenesis Experimental Design stimulus_or_stress_design replicate_design in_vitro_design co-expression_design transcription profiling by array Experimental Design Term Source REF mo mo mo EFO Comment[AEExperimentType] transcription profiling by array Comment[ArrayExpressReleaseDate] 2008-08-12 Comment[AEMIAMESCORE] 5 Comment[ArrayExpressAccession] E-MEXP-1467 Comment[MAGETAB TimeStamp_Version] 2010-08-09 21:59:54 Last Changed Rev: 13058 Experimental Factor Name dose compound Experimental Factor Type dose compound Experimental Factor Term Source REF Person Last Name Andres Person First Name German Person Mid Initials Person Email andres@med.unibs.it Person Phone 390303717318 Person Fax Person Address Viale Europa 11 Person Affiliation Biomedical Sciences Person Roles submitter Person Roles Term Source REF The MGED Ontology Quality Control Type biological_replicate Quality Control Term Source REF The MGED Ontology Replicate Type Replicate Term Source REF Normalization Type Normalization Term Source REF Date of Experiment Public Release Date 2008-08-12 PubMed ID 18624773 Publication DOI 18624773 Publication Author List Andrés G, Leali D, Mitola S, Coltrini D, Camozzi M, Corsini M, Belleri M, Hirsch E, Schwendener RA, Christofori G, Alcamí A, Presta M Publication Title A pro-inflammatory signature mediates FGF2-induced angiogenesis. Publication Status journal_article Publication Status Term Source REF The MGED Ontology Experiment Description Effect of FGF2 on the transcriptional profile of microvascular endothelial cells Protocol Name P-MEXP-137221 P-MEXP-137222 P-AFFY-2 Affymetrix:Protocol:Hybridization-EukGE-WS2v4 P-AFFY-6 P-MEXP-137789 Protocol Type specified_biomaterial_action nucleic_acid_extraction labeling hybridization feature_extraction bioassay_data_transformation Protocol Description "Murine lung microvascular 1G11 endothelial cells (Dong QG et al. ATVB 1997;17:1599) were stimulated for 10 hours with 30 ng/ml FGF-2 in DMEM supplemented with 0.5% inactivated fetal bovine serum after a 24-starvation period. As a control reference, non-stimulated cells were also analyzed." "Total RNA was extracted using Trizol (Invitrogen Life technologies, USA) and Phase lock gels (Eppendorf; Hamburg, Deutschland). RNA was further purified with Rneasy columns (Qiagen, Valencia, CA). Ten micrograms of purified RNA were used as a template for double-stranded cDNA synthesis primed using a T7-(dT)24 oligonucleotid according to standard Affymetrix protocol" Title: Affymetrix in vitro transcription. Description: Title: Fluidics Station Protocol. Description: Title: Affymetrix CEL analysis. Description: "Raw expression measures at the probe level data (Affy CEL files) were background adjusted, normalized, and log2-transformed using Robust Multi-array Average (RMA) and Affymetrix MOE430A CDF file. Quantile normalization was performed across all microarrays to achieve the same distribution of signal intensities for each array. Normalized expression data are expressed as log2-scale values." Protocol Parameters Amplification;Extracted product; Protocol Hardware Protocol Software MicroArraySuite 5.0 MicroArraySuite 5.0 Protocol Contact Protocol Term Source REF The MGED Ontology SDRF File E-MEXP-1467.sdrf.txt Term Source Name NCI_thesaurus The MGED Ontology ArrayExpress The MGED Ontology mo EFO Term Source File ncithesaurus.obo.alt http://mged.sourceforge.net/ontologies/MGEDontology.php http://www.ebi.ac.uk/arrayexpress http://mged.sourceforge.net/ontologies/MGEDontology.php http://mged.sourceforge.net/ontologies/MGEDontology.php http://www.ebi.ac.uk/efo/ Term Source Version