Investigation Title	Transcription profiling of human lung epithelial cells post-infected with P aeruginosa strains	
Comment[Submitted Name]	Pseudomonas aeruginosa infection of Calu-3 human lung epithelial cells	
Experimental Design	individual_genetic_characteristics_design	disease_state_design	transcription profiling by array	
Experimental Design Term Source REF		mo	EFO	
Comment[ArrayExpressReleaseDate]	2007-09-28	
Comment[SecondaryAccession]	GSE923	
Comment[AEMIAMESCORE]	4	
Comment[SecondaryAccession]	GDS858	
Comment[ArrayExpressAccession]	E-GEOD-923	
Comment[MAGETAB TimeStamp_Version]	2010-08-09 13:22:12 Last Changed Rev: 13058	
Experimental Factor Name	Genotype	DiseaseState	
Experimental Factor Type	genotype	disease_state	
Experimental Factor Term Source REF	
Person Last Name	Mychaleckyj	
Person First Name	Josyf	
Person Mid Initials	
Person Email	jmychale@wfubmc.edu	
Person Phone	
Person Fax	
Person Address	MYCHALECKYJ, Center for Human Genomics, Wake Forest Univ School of Medicine, Winston-Salem, 27157, USA	
Person Affiliation	Wake Forest Univ School of Medicine	
Person Roles	submitter	
Person Roles Term Source REF	The MGED Ontology	
Quality Control Type	
Quality Control Term Source REF	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2007-09-28	
PubMed ID	15494517	
Publication DOI	15494517	
Publication Author List	Laura M Cobb, Josyf C Mychaleckyj, Daniel J Wozniak, Yolanda S López-Boado	
Publication Title	Pseudomonas aeruginosa flagellin and alginate elicit very distinct gene expression patterns in airway epithelial cells: implications for cystic fibrosis disease.	
Publication Status	journal_article	
Publication Status Term Source REF	The MGED Ontology	
Experiment Description	This series contains 5 groups of U133A arrays with targets from Calu-3 cell line post-infection with P aeruginosa strains. Calu-3 human lung epithelial cells were seeded onto 6-well plates and grown to about 80% confluency. Epithelial monolayers were infected with 108 CFUs per ml of each bacterial strain for 60 min and then washed extensively with phosphate-buffered saline. The cultures were subsequently incubated in fresh RPMI 1640 medium supplemented with 10% fetal bovine serum and 100 µg/ml gentamicin. After 6 h, total RNA from the cells was prepared with RNAzol and further purified with RNEasy for microarray hybridization. All arrays processed and globally scaled to 500 using MAS 5. CONTROL (4 arrays) - no infection; FRD1 (4 arrays); FRD1234 (3 arrays); FRD440 (4 arrays); FRD875 (4 arrays)	
Protocol Name	
Protocol Type	
Protocol Description	
Protocol Parameters	
Protocol Hardware	
Protocol Software	
Protocol Contact	
Protocol Term Source REF	
SDRF File	E-GEOD-923.sdrf.txt	
Term Source Name	ncbitax		NCI_thesaurus	The MGED Ontology	ArrayExpress	mo	EFO	The MGED Ontology	
Term Source File	http://www.ncbi.nlm.nih.gov/Taxonomy/taxonomyhome.html		ncithesaurus.obo.alt	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	
Term Source Version