Comment[ArrayExpressAccession]	E-GEOD-84926
MAGE-TAB Version	1.1						
Public Release Date	2016-07-28
Investigation Title	Protein expression related with cell cycle control phosphorylation between TCCSUP and KSHV-infected TCCSUP						
Comment[Submitted Name]	Protein expression related with cell cycle control phosphorylation between TCCSUP and KSHV-infected TCCSUP
Experiment Description	Protein expression profile was analyzed by antibody array for cell cycle control phosphorylation with 238 antibodies with bladder cancer cell line, TCCSUP, and KSHV-infected TCCSUP cells. Protein was extracted from uninfected bladder cancer cell line, TCCSUP, and KSHV-infected TCCSUP cells, and they were analyzed by antibody array for cell cycle control phosphorylation.						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Lee	Lee	Park				
Person First Name	Myung-Shin	Myung	Jinsung				
Person Mid Initials		S					
Person Email	imslee@gmail.com						
Person Affiliation	Eulji University School of Medicine						
Person Phone	+82 42 259 1662						
Person Address	Microbiology and Immunology, Eulji University School of Medicine, Yongdu-dong, Jung-gu, Daejeon, South Korea						
Person Roles	submitter						
Protocol Name	P-GSE84926-1	P-GSE84926-5	P-GSE84926-6	P-GSE84926-2	P-GSE84926-3	P-GSE84926-4	P-GSE84926-7
Protocol Description	GenePix Software (v 6.1) was used for data extraction and Genowiz software (v 4.0.5.6) was used for beta-actin normalization. ID_REF =  VALUE = Normalized signal intensity	50ug of Tissue extract was labeled with Biotin and label buffer (Full Moon Bio).	Biotin-labeled extract was hybridized with antibody-spotted slide for 2hr at room temperature. After hybridization, slide was washed with 1X wash buffer (Full Moon Bio) and de-ionized water. Detection Buffer including Cy3-Streptavidin (Full Moon Bio) was treated for 20min at room temperature. Slide was washed with 1X wash buffer (Full Moon Bio) and de-ionized water.	Cells were cultured in T75 cell culture flask until confluency was about 80%. Then, protein lysates were collected by extracting buffer.	Both cells were cultured in EMEM with 10% FBS and 1% streptomycin/Penicillin under 5% CO2 and 37C incubator.	10~40mg of tissues were washed with cold 1X PBS. Tissue samples were homogenized with Extracting buffer (Full Moon Bio). Debris was removed from tissue extract with centrifuge.	Scanned on an Axon 4000B scanner.
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	virus						
Experimental Factor Type	virus						
Comment[SecondaryAccession]	GSE84926						
Comment[GEOReleaseDate]	2016-07-28						
Comment[ArrayExpressSubmissionDate]	2016-07-27						
Comment[GEOLastUpdateDate]	2016-07-30						
Comment[AEExperimentType]	proteomic profiling by array						
SDRF File	E-GEOD-84926.sdrf.txt