Comment[ArrayExpressAccession]	E-GEOD-74344
MAGE-TAB Version	1.1			
Public Release Date	2016-02-28
Investigation Title	Oocyte-expressed Yes-associated Protein (YAP) is a Key Activator of the Early Zygotic Genome in Mouse			
Comment[Submitted Name]	Oocyte-expressed Yes-associated Protein (YAP) is a Key Activator of the Early Zygotic Genome in Mouse
Experiment Description	We generated maternal and paternal mouse models with Yap1-deletion, and elucidated the function of maternal YAP in zygotic genome activation. RNA sequencing with 2 WT oocyte samples at GV stage, 2 YAP-/- oocyte samples at GV stage, 1 WT embryo samples at 4cell stage, 1 YAP-/- embryo samples at at 4-cell stage.			
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl		
Person Last Name	dang	Yu	Dang	
Person First Name	yujiao	Chao	Yujiao	
Person Email	1101110321@pku.edu.cn			
Person Affiliation	BIOPIC			
Person Address	BIOPIC, Yihe road, beijing, China			
Person Roles	submitter			
Protocol Name	P-GSE74344-4	P-GSE74344-1	P-GSE74344-3	P-GSE74344-2
Protocol Description	Illumina CASAVA version 1.8 were used to the basecalling. Reads were trimmed to remove the adapter sequences and low quality bases. RNA-seq reads were aligned to the mouse refference genome (mm9) using Tophat software gene expression level was calculated Cufflink. Genome_build: mm9 Supplementary_files_format_and_content: txt files reflect FPKM of each gene in Refseq.	Oocytes/embryos were collected from 21-23-day-old female WT and YAP-/- mice after injection of PMSG/hCG and mated for embryos.	60 oocytes/embryos were picked into the RLT lysis buffer, and RNA was prepared follow the protocol of QIAGEN's RNAeasy kit. Cells were picked into the lysis buffer to extracted mRNAs; then the purifed mRNAs were used to construct the cDNA library with the NEB RNA library construct kit. The quality-ensured libraries were used for pair-end deep sequencing on Illumina HiSeq Sequencer.	Mice were maintained under SPF conditions in a controlled environment of 20-22oC, with a 12/12 h light/dark cycle, 50-70% humidity, and food and water provided ad libitum. Animal care and experimental procedures were conducted in accordance with the Animal Research Committee guidelines of Zhejiang University.
Protocol Type	normalization data transformation protocol	sample treatment protocol	nucleic acid library construction protocol	growth protocol
Experimental Factor Name	developmental stage	oocyte genotype/variation	organism part	maternal mouse info
Experimental Factor Type	developmental stage	oocyte genotype/variation	organism part	maternal mouse info
Publication Title	Oocyte-expressed yes-associated protein is a key activator of the early zygotic genome in mouse.			
Publication Author List	Yu C, Ji SY, Dang YJ, Sha QQ, Yuan YF, Zhou JJ, Yan LY, Qiao J, Tang F, Fan HY			
PubMed ID	26902285			
Publication DOI	10.1038/cr.2016.20			
Comment[SecondaryAccession]	GSE74344			
Comment[GEOReleaseDate]	2016-02-28			
Comment[ArrayExpressSubmissionDate]	2015-10-26			
Comment[GEOLastUpdateDate]	2016-03-02			
Comment[AEExperimentType]	RNA-seq of coding RNA			
Comment[SecondaryAccession]	SRP065256			
Comment[SequenceDataURI]	http://www.ebi.ac.uk/ena/data/view/SRR2811581-SRR2811586			
SDRF File	E-GEOD-74344.sdrf.txt