Investigation Title	Transcription profiling of mouse leukocytes from blood and spleen after burn injury or trauma hemorrhage	
Comment[Submitted Name]	Comparison of Longitudinal Leukocyte Gene Expression after Burn Injury or Trauma Hemorrhage in Mice	
Experimental Design	unknown_experiment_design_type	transcription profiling by array	
Experimental Design Term Source REF		EFO	
Comment[AEMIAMESCORE]	4	
Comment[ArrayExpressReleaseDate]	2008-06-15	
Comment[SecondaryAccession]	GSE7404	
Comment[ArrayExpressAccession]	E-GEOD-7404	
Comment[MAGETAB TimeStamp_Version]	2010-08-07 22:42:39 Last Changed Rev: 13058	
Experimental Factor Name	
Experimental Factor Type	
Experimental Factor Term Source REF	
Person Last Name	the Host Response to Injury	
Person First Name	IDDC Inflammation	
Person Mid Initials	&.	
Person Email	data_curator@gluegrant.org	
Person Phone	
Person Fax	
Person Address	Information Dissemination and Data Coordination, Department of Molecular Biology, Massachusetts General Hospital, 38 Sidney St., Cambridge, 02139, USA	
Person Affiliation	Massachusetts General Hospital	
Person Roles	submitter	
Person Roles Term Source REF		
Quality Control Type	
Quality Control Term Source REF	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2008-06-15	
PubMed ID	
Publication DOI	
Publication Author List	
Publication Title	
Publication Status	
Publication Status Term Source REF	
Experiment Description	We report here the genes that are sequentially expressed in white blood cells from blood and spleen at 2 hours, 2 day,3 days, and 7 days after burn and sham injury or trauma-hemorrhage (T-H) and sham T-H. Experiment Overall Design: White blood cells (WBC) and splenocytes were harvested from individual mice and RNA was extracted, labeled and hybridized to Affymetrix Mouse Genome 430 2.0  GeneChips™. The results were analyzed by dCHIP and BRB Array Tools software. Ingenuity Pathway Analysis (IPA) was applied to identify molecular networks significantly affected by the injuries.	
Protocol Name	P-G7404-1	P-G7404-3	P-G7404-2	Affymetrix:Protocol:Hybridization-EukGE-WS2v4_450	P-AFFY-6	Affymetrix:Protocol:ExpressionStat	
Protocol Type	specified_biomaterial_action	nucleic_acid_extraction	labeling	hybridization	feature_extraction	bioassay_data_transformation	
Protocol Description	Leukocyte isolation from cardiac puncture followed by RBC lysis with proprietary buffer, followed by suspension in RLT buffer ;  Splenocytes in Dulbeco's PBS followed by the same RBC lysis RLT suspension process.	Total RNA was isolated using the manufacturer’s recommended protocol for RNeasy (Qiagen, Valencia, CA).	1-2 μg of total RNA was used to make single-stranded antisense cDNA using the NuGEN Technologies (San Carlo, CA) Ovation™ Biotin System in accordance with the manufacturer’s directions.	Title: Affymetrix EukGE-WS2v4_450 Hybridization. Description: 	Title: Affymetrix CEL analysis. Description: 	Title: Affymetrix CHP Analysis (ExpressionStat). Description: 	
Protocol Parameters	
Protocol Hardware	
Protocol Software					MicroArraySuite 5.0	MicroArraySuite 5.0	
Protocol Contact	
Protocol Term Source REF				mo	
SDRF File	E-GEOD-7404.sdrf.txt	
Term Source Name	The MGED Ontology	ArrayExpress	EFO	mo	
Term Source File	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	
Term Source Version