Investigation Title	Transcription profiling of E. coli covering four phases of growth in Fis KO and WT (Fis+) strains	
Comment[Submitted Name]	Expression data from E. coli covering four phases of growth in Fis KO and WT (Fis+) strains	
Experimental Design	unknown_experiment_design_type	transcription profiling by array	
Experimental Design Term Source REF		EFO	
Comment[ArrayExpressReleaseDate]	2008-06-15	
Comment[SecondaryAccession]	GSE7398	
Comment[AEMIAMESCORE]	4	
Comment[ArrayExpressAccession]	E-GEOD-7398	
Comment[MAGETAB TimeStamp_Version]	2010-08-07 18:56:36 Last Changed Rev: 13058	
Experimental Factor Name	
Experimental Factor Type	
Experimental Factor Term Source REF	
Person Last Name	Osuna	
Person First Name	Robert	
Person Mid Initials	
Person Email	osuna@albany.edu	
Person Phone	
Person Fax	
Person Address	Dr. Osuna, Biology, University at Albany, 1400 Washington Avenue, Albany, 12222, USA	
Person Affiliation	University at Albany	
Person Roles	submitter	
Person Roles Term Source REF		
Quality Control Type	
Quality Control Term Source REF	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2008-06-15	
PubMed ID	17768236	
Publication DOI	17768236	
Publication Author List	Meranda D Bradley, Michael B Beach, A P Jason de Koning, Timothy S Pratt, Robert Osuna	
Publication Title	Effects of Fis on Escherichia coli gene expression during different growth stages.	
Publication Status	journal_article	
Publication Status Term Source REF	The MGED Ontology	
Experiment Description	This SuperSeries is composed of the following subset Series:; GSE7379: Fis KO strain; GSE7380: WT (Fis+) strain Experiment Overall Design: Refer to individual Series	
Protocol Name	P-G7398-6	P-G7398-5	P-G7398-7	P-G7398-2	P-G7398-1	P-G7398-8	P-G7398-4	P-G7398-3	Affymetrix:Protocol:Hybridization-Unknown	P-AFFY-6	
Protocol Type	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	specified_biomaterial_action	grow	grow	nucleic_acid_extraction	labeling	hybridization	feature_extraction	
Protocol Description	Cells were grown in LB at 37 degrees celsius with shaking for 240 minutes	Cells were grown in LB at 37 degrees celsius with shaking for 150 minutes	Cells were grown in LB at 37 degrees celsius with shaking for 360 minutes	Cells were grown in LB at 37 degrees celsius with shaking for 90 minutes	Saturated E. coli cultures were diluted in LB toan OD.of  0.05 OD600, grown at 37 degrees Celsius	Saturated E. coli cultures were diluted in LB to an OD.of  0.05 OD600, grown at 37 degrees Celsius	MasterPure RNA purification Kit per manufacturers protocol	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).	Title: Affymetrix Generic Hybridization. Description: 	Title: Affymetrix CEL analysis. Description: 	
Protocol Parameters	
Protocol Hardware	
Protocol Software									MicroArraySuite 5.0	MicroArraySuite 5.0	
Protocol Contact	
Protocol Term Source REF									mo	
SDRF File	E-GEOD-7398.sdrf.txt	
Term Source Name	The MGED Ontology		ArrayExpress	EFO	The MGED Ontology	mo	
Term Source File	http://mged.sourceforge.net/ontologies/MGEDontology.php		http://www.ebi.ac.uk/arrayexpress	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://mged.sourceforge.net/ontologies/MGEDontology.php	
Term Source Version