Comment[ArrayExpressAccession] E-GEOD-73042 MAGE-TAB Version 1.1 Public Release Date 2016-07-22 Investigation Title Phenotypic, transcriptomic and genomic characterization of clonal plasma cells in light chain amyloidosis Comment[Submitted Name] Phenotypic, transcriptomic and genomic characterization of clonal plasma cells in light chain amyloidosis Experiment Description This SuperSeries is composed of the SubSeries listed below. Refer to individual Series Term Source Name ArrayExpress EFO Term Source File http://www.ebi.ac.uk/arrayexpress/ http://www.ebi.ac.uk/efo/efo.owl Person Last Name Paiva Person First Name Bruno Person Email bpaiva@unav.es Person Affiliation Clinica Universidad de Navarra Person Address Clinica Universidad de Navarra, Av. Pio XII, 55, Pamplona, Spain Person Roles submitter Protocol Name P-GSE73042-1 P-GSE73042-3 P-GSE73042-6 P-GSE73042-4 P-GSE73042-7 P-GSE73042-2 P-GSE73042-8 P-GSE73042-5 Protocol Description Expression value for each probe set was calculated using Affymetrix Expression Console that uses RMA (Robust Multi-Array Average) algorithm. HuGene-1_0-st-v1.r4.pgf HuGene-1_0-st-v1.r4.mps ID_REF = VALUE = Quantile normalized gene level expression values from Affymetrix Expression Console Labelling and hybridizations were performed according to protocols from Affymetrix. Briefly, 100-300 ng of total RNA were amplified and labeled using the WT Sense Target labelling and control reagents kit (Affymetrix Inc., Santa Clara, CA, USA) Labelling and hybridizations were performed according to protocols from Affymetrix Labeled RNA was hybridized to Human Gene 1.0 ST Array DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions. not provided The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000. Washing and scanning were performed using GeneChip System of Affymetrix (GeneChip Hybridization Oven 640, GeneChip Fluidics Station 450 and GeneChip Scanner 7G). Protocol Type normalization data transformation protocol labelling protocol labelling protocol hybridization protocol hybridization protocol nucleic acid extraction protocol array scanning protocol array scanning protocol Experimental Factor Name cell type diagnosis Experimental Factor Type cell type diagnosis Publication Title Phenotypic, transcriptomic, and genomic features of clonal plasma cells in light-chain amyloidosis. Publication Author List Paiva B, Martinez-Lopez J, Corchete LA, Sanchez-Vega B, Rapado I, Puig N, Barrio S, Sanchez ML, Alignani D, Lasa M, Garc�a de Coca A, Pardal E, Oriol A, Garcia ME, Escalante F, Gonz�lez-L�pez TJ, Palomera L, Alonso J, Prosper F, Orfao A, Vidriales MB, Mateos MV, Lahuerta JJ, Gutierrez NC, San Miguel JF PubMed ID 27069257 Publication DOI 10.1182/blood-2015-10-673095 Comment[SecondaryAccession] GSE73042 Comment[GEOReleaseDate] 2016-07-22 Comment[ArrayExpressSubmissionDate] 2015-09-15 Comment[GEOLastUpdateDate] 2016-07-22 Comment[AEExperimentType] transcription profiling by array Comment[AEExperimentType] comparative genomic hybridization by array SDRF File E-GEOD-73042.sdrf.txt