Comment[ArrayExpressAccession]	E-GEOD-72817
MAGE-TAB Version	1.1					
Public Release Date	2015-11-19
Investigation Title	Mytilus galloprovincialis, digestive gland and gill, exposed to Prorocentrum lima, producers of okadaic acid.					
Comment[Submitted Name]	Mytilus galloprovincialis, digestive gland and gill, exposed to Prorocentrum lima, producers of okadaic acid.
Experiment Description	Differential expression analysis of digestive gland and gill tissues of mussels (Mytilus galloprovincialis) exposed to dinoflagellates (Prorocentrum lima), producers of okadaic acid, at a concentration of 200 cells/ml for one day. Each sample consists in total RNA was extracted from pooled tissues of 5 individuals. Two-color dye-swap direct comparison experiment: exposed vs non-exposed (treated vs control). Biological replicates: two treated replicates and two control replicates per tissue.					
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl				
Person Last Name	Fernandez Tajes	Fernandez-Tajes				
Person First Name	Juan	Juan				
Person Email	jfernandezt@udc.es					
Person Affiliation	INIBIC					
Person Address	Genomics Area, INIBIC, As Xubias, A Coruña, Spain					
Person Roles	submitter					
Protocol Name	P-GSE72817-1	P-GSE72817-4	P-GSE72817-5	P-GSE72817-2	P-GSE72817-3	P-GSE72817-6
Protocol Description	Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1) ID_REF =  VALUE = Background corrected and normalized with loess and Aquantile log2 ratio (Cy5/Cy3)	Two-color microarray-based LowInput QuickAmp Labeling (Agilent)	Microarrays were hybridized and washed according to manufacturer's protocol (version 6.5).	Specimens were collected from the natural environment in an area free of dinoflagellate bloom at the time of collection; Valcobo beach, Galicia, Spain. Sampled individuals were randomly divided into two groups; exposed to Prorocentrum lima (treated group) and non-exposed (control group). Both groups were kept in aerated tanks of seawater and fed with a combined diet of Tetraselmis suecica and Isochrysis galbana microalgae. After acclimation, the exposed group was fed with 200 cells/ml of a P. lima culture in exponential phase for 24 hours.	Total RNA was extracted from pooled tissue of 5 individuals per group using Trizol according to manufacturer's instructions	Scanned on an Agilent G2505C scanner
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	treatment					
Experimental Factor Type	treatment					
Comment[SecondaryAccession]	GSE72817					
Comment[GEOReleaseDate]	2015-11-19					
Comment[ArrayExpressSubmissionDate]	2015-09-09					
Comment[GEOLastUpdateDate]	2015-11-21					
Comment[AEExperimentType]	transcription profiling by array					
SDRF File	E-GEOD-72817.sdrf.txt