Comment[ArrayExpressAccession]	E-GEOD-72815
MAGE-TAB Version	1.1		
Public Release Date	2015-09-09
Investigation Title	Effects of Age and Estrogen on Skeletal Gene Expression in Humans as Assessed by RNA Sequencing		
Comment[Submitted Name]	Effects of Age and Estrogen on Skeletal Gene Expression in Humans as Assessed by RNA Sequencing
Experiment Description	Here we present an RNAseq analysis of human bone samples, obtained from iliac crest needle biopsies, to yield the first in vivo interrogation of all genes and pathways that may be altered in bone with aging and E therapy in humans. 58 healthy women were studied, including 19 young women (mean age ± SD, 30.3 ± 5.4 years), 19 old women (73.1 ± 6.6 years), and 20 old women treated with 3 weeks of E therapy (70.5 ± 5.2 years).  Using generally accepted criteria (false discovery rate [q] < 0.10), aging altered a total of 678 genes and 12 pathways, including a subset known to regulate bone metabolism (e.g., Notch).		
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl	
Person Last Name	Khosla	Khosla	Monroe
Person First Name	Sundeep	Sundeep	David
Person Mid Initials			G
Person Email	geo@ncbi.nlm.nih.gov		
Person Affiliation	Mayo Clinic		
Person Address	Endocrinology Research, Mayo Clinic, 200 1st St SW, Rochester, MN, USA		
Person Roles	submitter		
Protocol Name	P-GSE72815-1		
Protocol Description	we obtained small needle bone biopsies from the posterior iliac crest of all subjects using an 8G needle under local anesthesia total RNA was isolated using the RNeasy Micro Kit (Qiagen, Valencia, CA) and treated with the Turbo DNA-freeTM Kit (Life Technologies, Grand Island, NY) to remove potential contaminating DNA that may lead to false-positive amplification		
Protocol Type	nucleic acid library construction protocol		
Experimental Factor Name	age		
Experimental Factor Type	age		
Comment[SecondaryAccession]	GSE72815		
Comment[GEOReleaseDate]	2015-09-09		
Comment[ArrayExpressSubmissionDate]	2015-09-08		
Comment[GEOLastUpdateDate]	2015-09-09		
Comment[AEExperimentType]	RNA-seq of coding RNA		
Comment[AdditionalFile:Data1]	GSE72815_YOE_counts.csv		
Comment[SecondaryAccession]	SRP063477		
SDRF File	E-GEOD-72815.sdrf.txt