Comment[ArrayExpressAccession]	E-GEOD-72001
MAGE-TAB Version	1.1			
Public Release Date	2015-10-26
Investigation Title	RNA-seq following enhancer repression			
Comment[Submitted Name]	RNA-seq following enhancer repression
Experiment Description	Using RNA-seq to identify genes regulated by dCas9-KRAB mediated enhancer repression in NCI-H2009 lung adenocarcinoma cells NCI-H2009 cells were first transfected with dCas9-KRAB fusion and subsequently transfected with no guide RNA (Empty), a guide RNA that has no target in the human genome (Dummy), and two separate guide RNAs (e3 #1 and e3 #2) recognizing the enhancer region of our study.			
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl		
Person Last Name	Zhang	Zhang	Francis	
Person First Name	Xiaoyang	Xiaoyang	Joshua	
Person Email	geo@ncbi.nlm.nih.gov			
Person Affiliation	Dana-Farber Cancer Institute			
Person Address	Dana-Farber Cancer Institute, 450 Brookline Ave, Boston, MA, USA			
Person Roles	submitter			
Protocol Name	P-GSE72001-4	P-GSE72001-1	P-GSE72001-3	P-GSE72001-2
Protocol Description	Prada alignment Cufflinks normalization Genome_build: hg19 Supplementary_files_format_and_content: gct, normalized expression level for each gene	Cells were ransfected with the dCas9-KRAB fustion and subsequently transfected with guide RNAs	RNA was extracted using Qiagen RNeasy kit (74104) and mRNA was subsequently isolated using NEB poly(A) module (E7490) Following NEB RNA-seq library preparation kit (E7420S) Following NEB RNA-seq library preparation kit (E7420S)	Cells were grown in RPMI medium supplemented with 10% FBS
Protocol Type	normalization data transformation protocol	sample treatment protocol	nucleic acid library construction protocol	growth protocol
Experimental Factor Name	treatment			
Experimental Factor Type	treatment			
Comment[SecondaryAccession]	GSE72001			
Comment[GEOReleaseDate]	2015-10-26			
Comment[ArrayExpressSubmissionDate]	2015-08-12			
Comment[GEOLastUpdateDate]	2015-10-26			
Comment[AEExperimentType]	RNA-seq of coding RNA			
Comment[AdditionalFile:Data1]	GSE72001_KRAB_rpkm.gct			
Comment[SecondaryAccession]	SRP062332			
Comment[SequenceDataURI]	http://www.ebi.ac.uk/ena/data/view/SRR2156846-SRR2156853			
SDRF File	E-GEOD-72001.sdrf.txt