Comment[ArrayExpressAccession]	E-GEOD-70528
MAGE-TAB Version	1.1											
Public Release Date	2015-07-07
Investigation Title	Gene expression annalysis of peripheral blood cells in patients with chronic kidney disease											
Comment[Submitted Name]	Gene expression annalysis of peripheral blood cells in patients with chronic kidney disease
Experiment Description	Genes related to sleep and wakefulness were evaluated by RNA microarray in patients, including CKD,HD patients and control subjects. RNA microarray analysis was performed in 19 patients without diabetes. The patients were divided into three groups: HT group (8 hypertension patients with eGFR of >60 mL/min/1.73 m2), CKD group (7 patients not on dialysis with eGFR of <60 mL/min/1.73 m2) and HD group (4 HD patients).											
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl										
Person Last Name	Kitajima	Kitajima	Iwata	Furuichi	Sagara	Shinozaki	Toyama	Sakai	Shimizu	Sakurai	Kaneko	Wada
Person First Name	Shinji	Shinji	Yasunori	Kengo	Akihiro	Yasuyuki	Tadashi	Norihiko	Miho	Takeshi	Shuichi	Takashi
Person Email	geo@ncbi.nlm.nih.gov											
Person Affiliation	Kanazawa University hospital											
Person Address	Kanazawa University hospital, 13-1 Takara-machi, Kanazawa, Japan											
Person Roles	submitter											
Protocol Name	P-GSE70528-1	P-GSE70528-5	P-GSE70528-6	P-GSE70528-2	P-GSE70528-3	P-GSE70528-4	P-GSE70528-7					
Protocol Description	Data was extracted using the GeneChip scanner 3000 (Affymetrix, Santa Clara, CA) and raw data file formats were generated using the Gene Chip Operating Software (GCOS). ID_REF =  VALUE = Normalized intensity	After the second cycle cDNA synthesis, biotin-labelled cRNA was generated using the Affymetrix IVT labelling kit	The labelled cRNA was cleaned, quantified and hybridized to Affymetrix HG-U133 Plus2.0 GeneChips after fragmentation.	RNA was extracted from whole blood using the PAXgene Blood RNA System Kit	Blood specimens (2.5 ml) were collected from AM 8 to 10’, before hemodialysis in PAXgene tubes from each patient.	Total RNA (100 ng) was used for the generation of biotin-labelled cRNA using the Affymetrix Two-Cycle cDNA Synthesis Kit (Affymetrix, Santa Clara, CA)	The chips were washed and stained with streptavidin phycoerythrin was amplified using a biotinylated antibody solution in an Affymetrix Fluidics Station 450 according to the Affymetrix GeneChip protocol.					
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol					
Experimental Factor Name	sex											
Experimental Factor Type	sex											
Comment[SecondaryAccession]	GSE70528											
Comment[GEOReleaseDate]	2015-07-07											
Comment[ArrayExpressSubmissionDate]	2015-07-06											
Comment[GEOLastUpdateDate]	2015-07-08											
Comment[AEExperimentType]	transcription profiling by array											
SDRF File	E-GEOD-70528.sdrf.txt