Source Name Comment [Sample_characteristics] Comment [Sample_description] Comment [Sample_source_name] Characteristics[Organism] Term Source REF Term Accession Number Characteristics[antibody] Characteristics[Antibody] Characteristics[Organism] Term Source REF Term Accession Number Extract Name Material Type Assay Name Technology Type Comment [Platform_title] Protocol REF Term Source REF Protocol REF Term Source REF Normalization Name Derived Array Data File Comment [Derived ArrayExpress FTP file] GSM160698 1 human primary CD3+ T cell (resting) The GMAT (Genome-wide MApping Technique) was developed by the combination of ChIP (chromatin immunoprecipitation) and SAGE(serial analysis of gene expression) protocols. Briefly, the chromatin prepared by sonication is subject to immunoprecipitation using specific antibody. DNA from precipitated chromatin is ligated with biotinylated linker and digested with NlaIII restriction enzyme. The DNA fragments with biotinylated linker are isolated and ligated with NlaIII-site specific linker. The DNA is cut with MmeI restriction enzyme to generate 21-base seqeunce tags and ligated each other to form ditags. After removing linkers by NlaIII, isolated ditags are concatenated and determined the sequence. The genomic position of tags is identified by DNA sequences and the detected number of each tag sequence represents the degree of enrichment by means of specific antibody. Human_R_T_H3ac Homo sapiens EFO http://purl.org/obo/owl/NCBITaxon#NCBITaxon_9606 histone H3 diaceylation at K9 and K14 GSM160698 extract 1 genomic DNA GSM160698 assay GMAT (Genome-wide MAppping Technique) [NlaIII: Homo Sapiens] tag list P-GSE6974-1 P-GSE6974-2 GSM160698_sample_table.txt norm GSM160698_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-6974/E-GEOD-6974.processed.1.zip GSM160695 1 human primary CD3+ T cell (activated with anti-CD3 and anti-CD28 antibodies (1ug/ml) for 24 hr) The GMAT (Genome-wide MApping Technique) was developed by the combination of ChIP (chromatin immunoprecipitation) and SAGE(serial analysis of gene expression) protocols. Briefly, the chromatin prepared by sonication is subject to immunoprecipitation using specific antibody. DNA from precipitated chromatin is ligated with biotinylated linker and digested with NlaIII restriction enzyme. The DNA fragments with biotinylated linker are isolated and ligated with NlaIII-site specific linker. The DNA is cut with MmeI restriction enzyme to generate 21-base seqeunce tags and ligated each other to form ditags. After removing linkers by NlaIII, isolated ditags are concatenated and determined the sequence. The genomic position of tags is identified by DNA sequences and the detected number of each tag sequence represents the degree of enrichment by means of specific antibody. Human_A_T_H3ac histone H3 diaceylation at K9 and K14 Homo sapiens EFO http://purl.org/obo/owl/NCBITaxon#NCBITaxon_9606 GSM160695 extract 1 genomic DNA GSM160695 assay GMAT (Genome-wide MAppping Technique) [NlaIII: Homo Sapiens] tag list P-GSE6974-1 P-GSE6974-2 GSM160695_sample_table.txt norm GSM160695_sample_table.txt ftp://ftp.ebi.ac.uk/pub/databases/microarray/data/experiment/GEOD/E-GEOD-6974/E-GEOD-6974.processed.1.zip