Investigation Title	Transcription profiling of human coxsackievirus B3 (CVB3) infected HeLa cells (multiple time points and triplicate sample per time point)	
Comment[Submitted Name]	CVB3-infected HeLa cells (multiple time points and triplicate sample per time point)	
Experimental Design	time_series_design	disease_state_design	compound_treatment_design	co-expression_design	transcription profiling by array	
Experimental Design Term Source REF	mo	mo	mo	mo	EFO	
Comment[ArrayExpressReleaseDate]	2007-09-28	
Comment[SecondaryAccession]	GSE697	
Comment[AEMIAMESCORE]	3	
Comment[SecondaryAccession]	GDS478	
Comment[ArrayExpressAccession]	E-GEOD-697	
Comment[MAGETAB TimeStamp_Version]	2010-08-07 17:02:14 Last Changed Rev: 13058	
Experimental Factor Name	DiseaseState	Time	Compound	
Experimental Factor Type	disease_state	time	compound	
Experimental Factor Term Source REF	
Person Last Name	McManus	
Person First Name	Bruce Maxwell	
Person Mid Initials	
Person Email	bmcmanus@mrl.ubc.ca	
Person Phone	
Person Fax	
Person Address	Cardiovascular Research Laboratory, Department of Pathology and Laboratory Medicine, iCAPTURE CENTRE Room 166, St. Paul's Hospital, 1081 Burrard Street, Vancouver, V6Z 1Y6, Canada	
Person Affiliation	iCAPTURE CENTRE Room 166, St. Paul's Hospital	
Person Roles	submitter	
Person Roles Term Source REF		
Quality Control Type	
Quality Control Term Source REF	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2007-09-28	
PubMed ID	
Publication DOI	
Publication Author List	
Publication Title	
Publication Status	
Publication Status Term Source REF	
Experiment Description	HeLa cells were serum starved and either preincubated with DMSO (vehicle), preincubated with U0126 (10µM in DMSO) and infected with CVB3 (MOI 10), or preincubated with DMSO (vehicle) and infected with CVB3.  Following infection, virus was removed and fresh media containing 10% fetal bovine serum was added for the remainder of the infectious process.  At 0, 30 minutes, 3 and 9 hours following CVB3 infection (N=3 plates per time point), RNA was isolated, processed and hybridized to GeneChip®s (N=3 per sample)	
Protocol Name	P-GEOD-697-1	
Protocol Type	specified_biomaterial_action	
Protocol Description		
Protocol Parameters	
Protocol Hardware	
Protocol Software	
Protocol Contact	
Protocol Term Source REF	
SDRF File	E-GEOD-697.sdrf.txt	
Term Source Name	ncbitax	The MGED Ontology	ArrayExpress	NCI_thesaurus		mo	EFO	
Term Source File	http://www.ncbi.nlm.nih.gov/Taxonomy/taxonomyhome.html	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	ncithesaurus.obo.alt		http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/efo/	
Term Source Version