Comment[ArrayExpressAccession]	E-GEOD-66917
MAGE-TAB Version	1.1																			
Public Release Date	2015-07-09
Investigation Title	Leucegene: AML sequencing (part 4)																			
Comment[Submitted Name]	Leucegene: AML sequencing (part 4)
Experiment Description	RNA sequencing of human leukemia The goals of this project are to obtain a comprehensive study of mutations and gene expression in human acute myeloid leukemia (AML). Methods: AML cells were thawed. DNA and RNA (polyA) was extracted and sequences were obtained with an illumina HiSeq 2000 sequencer. Results are pending.																			
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl																		
Person Last Name	Sauvageau	Lavallée	Krosl	Wilhelm	Barabé	Gendron	Boucher	Lambert	Rondeau	Bilodeau	Lavallée	Pabst	Yeh	Lehnertz	Johnson	Thiollier	D'Angelo	Lemieux	Hébert	Sauvageau
Person First Name	Guy	Vincent	Jana	Brian	Frédéric	Patrick	Geneviève	Raphaëlle	Claude	Annie	Sylvie	Caroline	Jonathan	Bernhard	Radia	Clarisse	Giovanni	Sébastien	Josée	Guy
Person Email	geo@ncbi.nlm.nih.gov																			
Person Affiliation	Institute for Research in Immunology and Cancer																			
Person Address	Institute for Research in Immunology and Cancer, 2950, Chemin de Polytechnique, Montreal, QC, Canada																			
Person Roles	submitter																			
Protocol Name	P-GSE66917-2	P-GSE66917-1																		
Protocol Description	Basecalls performed using CASAVA 1.8.2 Sequenced reads were masked for sequencing adapters and mapped to the reference genome using CASAVA 1.8.2 Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using CASAVA 1.8.2 Genome_build: hg19 Supplementary_files_format_and_content: Tab-delimited text files include RPKM values for each sample. Columns are: Chromosome, Start location, Stop location, Gene, RPKM, base count.	TriZol + RNeasy mini Kit (Qiagen, #74106) TruSeq RNA Sample Preparation (Illumina)																		
Protocol Type	normalization data transformation protocol	nucleic acid library construction protocol																		
Experimental Factor Name	organism part																			
Experimental Factor Type	organism part																			
Comment[SecondaryAccession]	GSE66917																			
Comment[GEOReleaseDate]	2015-07-09																			
Comment[ArrayExpressSubmissionDate]	2015-03-16																			
Comment[GEOLastUpdateDate]	2015-07-16																			
Comment[AEExperimentType]	RNA-seq of coding RNA																			
Comment[SecondaryAccession]	SRP056197																			
Comment[SequenceDataURI]	http://www.ebi.ac.uk/ena/data/view/SRR1916263-SRR1916343																			
SDRF File	E-GEOD-66917.sdrf.txt