Comment[ArrayExpressAccession]	E-GEOD-65666
MAGE-TAB Version	1.1					
Public Release Date	2015-08-06
Investigation Title	Expression profile in Overexpression of GST,GST-Med3					
Comment[Submitted Name]	Expression profile in Overexpression of GST,GST-Med3
Experiment Description	We have used microarrays to study the gene expression changes in Overexpression of GST,GST-Med3  of yeast cells to detect how the overexpressioin may affect global  gene expression. RNA samples were taken from yeast cells overexpressed GST or GST-med3 and hybridized to affymetrix microarrays: GST and GST-Med3					
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl				
Person Last Name	Gustafsson	Zhu				
Person First Name	Claes	Xuefeng				
Person Email	claes.gustafsson@medkem.gu.se					
Person Affiliation	Gothenburg University					
Person Address	Department of Medical biochemistry and cell biology, Gothenburg University, Medcinaregatan 9A, Gothenburg, Sweden					
Person Roles	submitter					
Protocol Name	P-GSE65666-1	P-GSE65666-4	P-GSE65666-5	P-GSE65666-2	P-GSE65666-3	P-GSE65666-6
Protocol Description	Raw data (CEL files) was imported into ArrayStar 4, per-gene normalized to median and filtered on flags. ID_REF =  VALUE = plier summary normalized	Labeling was performed according to the GeneChip expression analysis technical manual that is available for download at www.affymetrix.com.	According to the GeneChip expression analysis technical manual that is available for download at www.affymetrix.com.	Yeast cells were grown SC-ura plus galactose medium	RNA was isolated from yeast cells according to Xue et al. 2004 (Yeast 21(1):25-39).	According to the GeneChip expression analysis technical manual that is available for download at www.affymetrix.com.
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	overexpression					
Experimental Factor Type	overexpression					
Publication Title	Mediator tail subunits can form amyloid-like aggregates in vivo and affect stress response in yeast.					
Publication Author List	Zhu X, Chen L, Carlsten JO, Liu Q, Yang J, Liu B, Gustafsson CM					
PubMed ID	26138482					
Publication DOI	10.1093/nar/gkv629					
Comment[SecondaryAccession]	GSE65666					
Comment[GEOReleaseDate]	2015-08-06					
Comment[ArrayExpressSubmissionDate]	2015-02-05					
Comment[GEOLastUpdateDate]	2015-08-06					
Comment[AEExperimentType]	transcription profiling by array					
SDRF File	E-GEOD-65666.sdrf.txt