Comment[ArrayExpressAccession]	E-GEOD-63215
MAGE-TAB Version	1.1				
Public Release Date	2015-07-28
Investigation Title	An RNAi screen probing signaling control of human embryonic stem cells establishes the cell cycle-regulated restriction of the exit from pluripotency (CCNB1 overexpression)				
Comment[Submitted Name]	An RNAi screen probing signaling control of human embryonic stem cells establishes the cell cycle-regulated restriction of the exit from pluripotency (CCNB1 overexpression)
Experiment Description	Analysis of effect of Cyclin B1 overexpression on differentiating hESCs at the gene expression level. The hypothesis tested in the present study was that Cyclin B1 overexpression prevents the exit from pluripotency in hESCs. Results provide important information on the effect of Cyclin B1 on hESCs, such as changes in expression of TGFb-related genes and pluripotency markers, as well as mesodermic and neuroectodermic genes. Total RNA obtained from CCNB1 overexpressing hESCs incubated in medium without bFGF and TGF-beta for 0, 48 and 96 hours.				
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl			
Person Last Name	Gonzales				
Person First Name	Kevin Andrew				
Person Email	a0079796@nus.edu.sg				
Person Affiliation	Genome Institute of Singapore				
Person Address	Genome Institute of Singapore, 60 Biopolis Street, Singapore, Singapore				
Person Roles	submitter				
Protocol Name	P-GSE63215-1	P-GSE63215-3	P-GSE63215-4	P-GSE63215-2	P-GSE63215-5
Protocol Description	Data were rank invariant normalized using BeadStudio ID_REF =  VALUE = Rank invariant normalized Detection Pval = 	Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification Kit	Standard Illumina hybridization protocol	RNA was extracted with Trizol reagent, followed by Dnase I treatment and clean-up with PureLink RNA Mini kit in accordance with the prescribed protocol provided with the kit.	Standard Illumina scanning protocol
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	treatment length	treatment type			
Experimental Factor Type	treatment length	treatment type			
Comment[SecondaryAccession]	GSE63215				
Comment[GEOReleaseDate]	2015-07-28				
Comment[ArrayExpressSubmissionDate]	2014-11-12				
Comment[GEOLastUpdateDate]	2015-07-29				
Comment[AEExperimentType]	transcription profiling by array				
Comment[AdditionalFile:Data1]	GSE63215_10001322020.sdf				
Comment[AdditionalFile:Data2]	GSE63215_10001322020_Metrics.txt				
Comment[AdditionalFile:Data3]	GSE63215_10001322020_fiducial__1_Red_center.tif				
Comment[AdditionalFile:Data4]	GSE63215_10001322020_fiducial__2_Red_center.tif				
Comment[AdditionalFile:Data5]	GSE63215_10001322020_fiducial__3_Red_center.tif				
Comment[AdditionalFile:Data6]	GSE63215_10001322020_normalized.txt				
Comment[AdditionalFile:Data7]	GSE63215_10001322027.sdf				
Comment[AdditionalFile:Data8]	GSE63215_10001322027_Metrics.txt				
Comment[AdditionalFile:Data9]	GSE63215_10001322027_fiducial__1_Red_center.tif				
Comment[AdditionalFile:Data10]	GSE63215_10001322027_fiducial__2_Red_center.tif				
Comment[AdditionalFile:Data11]	GSE63215_10001322027_fiducial__3_Red_center.tif				
Comment[AdditionalFile:Data12]	GSE63215_10001322027_normalized.txt				
SDRF File	E-GEOD-63215.sdrf.txt