Comment[ArrayExpressAccession]	E-GEOD-61314
MAGE-TAB Version	1.1						
Public Release Date	2014-09-11
Investigation Title	Cell population kinetics of collagen scaffolds in ex vivo oral wound repair						
Comment[Submitted Name]	Cell population kinetics of collagen scaffolds in ex vivo oral wound repair
Experiment Description	This study sought to provide a novel ex vivo model for analyzing healing kinetics and gene expression of primary human gingival fibroblasts (hGF) within collagen scaffolds. Sponge type and gel type scaffolds with and without platelet-derived growth factor-BB (PDGF) were assessed in an hGF containing matrix. Four samples were analyzed; gel w/ and w/o PDGF and scaffold w/ and w/o PDGF						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Giannobile	Giannobile	Gorlach	Agis			
Person First Name	William	William	Christoph	Hermann			
Person Mid Initials		V					
Person Email	wgiannob@umich.edu						
Person Affiliation	University of Michigan						
Person Address	Periodontics and Oral Medicine, University of Michigan, 1011 N. University Ave., Ann Arbor, Michigan, USA						
Person Roles	submitter						
Protocol Name	P-GSE61314-1	P-GSE61314-5	P-GSE61314-6	P-GSE61314-2	P-GSE61314-3	P-GSE61314-4	P-GSE61314-7
Protocol Description	The data were analyzed with Expression Console. ID_REF =  VALUE = Signal ABS_CALL = indicating whether the transcript was present (P), absent (A), or marginal (M) DETECTION P-VALUE = 	Biotinylated cRNA were prepared according to the standard Affymetrix protocol	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.	Collagen scaffolds and gel (w/ and w/o PDGF-BB) was overlaid with HGF cells in BME and harvested after 1-14 days for RNA extraction	HGF cells were suspended in BME with 1% FBS	Trizol method of RNA extraction was performed to manufacturer's instructions.	GeneChips were scanned using the Scanner 3000 7G.
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	SCAFFOLD	GROWTH FACTOR					
Experimental Factor Type	scaffold	growth factor					
Comment[SecondaryAccession]	GSE61314						
Comment[GEOReleaseDate]	2014-09-11						
Comment[ArrayExpressSubmissionDate]	2014-09-10						
Comment[GEOLastUpdateDate]	2014-09-11						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-61314.sdrf.txt