Comment[ArrayExpressAccession]	E-GEOD-60541
MAGE-TAB Version	1.1						
Public Release Date	2014-08-21
Investigation Title	Exposure of radiation on mouse lung						
Comment[Submitted Name]	Exposure of radiation on mouse lung
Experiment Description	This study was designed to identify molecular changes induced by radiation in mouse lung. Mouse left lung was irradiated with a single dose of radiation. Total RNA from the irradiated lung tissue was subject to single channnel microarray.						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Kim	Kim	Lee	Jin	Kang	Cho	Lee
Person First Name	Bu-Yeo	Bu-Yeo	Yoon-Jin	Hee	Ga-Young	Jaeho	Yun-Sil
Person Email	geo@ncbi.nlm.nih.gov						
Person Affiliation	Korea Institute of Oriental Medicine						
Person Address	Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon, South Korea						
Person Roles	submitter						
Protocol Name	P-GSE60541-1	P-GSE60541-5	P-GSE60541-6	P-GSE60541-2	P-GSE60541-3	P-GSE60541-4	P-GSE60541-7
Protocol Description	Signal intensities of genes were normalized by the quantile method. ID_REF =  VALUE = Quantile normalization, median subtraction	The pooled RNA was amplified and labeled using AgilentM-bM-^@M-^Ys Low RNA Input Linear Amplification kit PLUS (Agilent Technologies, USA).	The labelled RNA were hybridized to a microarray (Agilent Rat whole genome 44K, Agilent Technologies, USA) containing approximately 44,000 probes (~26,600 unique genes) in accordance with the manufacturer's instructions.	During irradiation, the mice were anesthetized with an intraperitoneally administered mixture of 30 mg/kg of zoletil and 10 mg/kg of rompun.	The mice were housed in a room with the temperature controlled at 24M-11 M-0C at a relative humidity of 50M-110%, with a 12h dark/light cycle. Food and water were provided ad libitum throughout the experiment.	Total RNA from mouse lung tissues was prepared using the Easy-SpinTM total RNA extraction kit according to the manufacturerM-bM-^@M-^Ys instructions (iNtRON Biotechnology, Seoul, Republic of Korea).	Arrays were scanned with an Agilent DNA Microarray Scanner.
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Comment[SecondaryAccession]	GSE60541						
Comment[GEOReleaseDate]	2014-08-21						
Comment[ArrayExpressSubmissionDate]	2014-08-20						
Comment[GEOLastUpdateDate]	2014-08-21						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-60541.sdrf.txt