Comment[ArrayExpressAccession]	E-GEOD-58943
MAGE-TAB Version	1.1				
Public Release Date	2014-07-20
Investigation Title	T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination - 2009				
Comment[Submitted Name]	T cell responses to H1N1v and a longitudinal study of seasonal influenza vaccination - 2009
Experiment Description	Systems biology approach to examine effects of seasonal flu vaccination in adults of different ages on gene expression, cytokine stimulation and serum cytokines with parameters such as immune senescence to uncover new markers and mechanisms behind failure of immune function in many older people. Total RNA was extracted from whole-blood lysates obtained from individuals immediately prior to vaccination against seasonal influenza, followed by depletion of globin messenger RNA. The goal was to evaluate potential gene expression signatures at Day 0 associated with immune senescence.				
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl			
Person Last Name	Furman	Davis			
Person First Name	David	Mark			
Person Mid Initials		M			
Person Email	furmand@stanford.edu				
Person Affiliation	Stanford University				
Person Address	Microbiology and Immunolgy, Stanford University, 279 Campus Dr, B226, Stanford, USA				
Person Roles	submitter				
Protocol Name	P-GSE58943-1	P-GSE58943-3	P-GSE58943-4	P-GSE58943-2	P-GSE58943-5
Protocol Description	Data was analyzed by Illumina's GenomeStudio software, version 2.0. Normalized values are raw values normalized to the overall intensity to the median average intensity for all samples across multiple arrays and chips after background normalization. ID_REF =  VALUE = normalized signals Detection Pval = 	For each sample, 750 ng of total RNA were hybridized to Beadchips (HumanHT-12v3 Expression Bead Chip, Illumina, San Diego, CA)	Standard illumina protocol	Total RNA was extracted from PAXgene blood RNA tubes (PreAnalytiX, USA) using the QIAcube automation RNA extraction procedure according to the manufacturer s protocol (Qiagene, Valencia, CA).	The hybridized Beadchips were scanned on an Illumina BeadScan confocal scanner
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	RACE	SEX			
Experimental Factor Type	race	Sex			
Comment[SecondaryAccession]	GSE58943				
Comment[GEOReleaseDate]	2014-07-20				
Comment[ArrayExpressSubmissionDate]	2014-06-30				
Comment[GEOLastUpdateDate]	2014-07-23				
Comment[AEExperimentType]	transcription profiling by array				
Comment[AdditionalFile:Data1]	GSE58943_non-normalized.txt				
SDRF File	E-GEOD-58943.sdrf.txt