Investigation Title	Transcription profiling of Arabidopsis to identify gene expression during silique senescence				
Comment[Submitted Name]	To identify changes in gene expression during silique senescence in Arabidopsis thaliana				
Experimental Design	unknown_experiment_design_type	transcription profiling by array			
Experimental Design Term Source REF		EFO			
Comment[AEExperimentType]	transcription profiling by array				
Comment[AEExperimentDisplayName]	Transcription profiling by array of Arabidopsis young and old siliques				
Comment[AEMIAMESCORE]	4				
Comment[ArrayExpressReleaseDate]	2008-06-14				
Comment[SecondaryAccession]	GSE5736				
Comment[ArrayExpressAccession]	E-GEOD-5736				
Comment[MAGETAB TimeStamp_Version]	2010-08-06 17:27:33 Last Changed Rev: 13058				
Experimental Factor Name	SILIQUE AGE				
Experimental Factor Type	age				
Experimental Factor Term Source REF					
Person Last Name	(NASC)				
Person First Name	Nottingham Arabidopsis Stock Centre				
Person Mid Initials					
Person Email	affy@arabidopsis.info				
Person Phone					
Person Fax					
Person Address	School of Biosciences, University of Nottingham, Nottingham Arabidopsis Stock Centre (NASC), Sutton Bonington Campus, Loughborough, LE12 5RD, United Kingdom				
Person Affiliation	Nottingham Arabidopsis Stock Centre (NASC)				
Person Roles	submitter				
Person Roles Term Source REF					
Quality Control Type					
Quality Control Term Source REF					
Replicate Type					
Replicate Term Source REF					
Normalization Type					
Normalization Term Source REF					
Date of Experiment					
Public Release Date	2008-06-14				
PubMed ID					
Publication DOI					
Publication Author List					
Publication Title					
Publication Status					
Publication Status Term Source REF					
Experiment Description	The aim of the experiment is to compare and contrast the profiles of gene expression during silique senescence and to identify the molecular events that regulate the timing of the process. Silique occurs over a highly predictable time frame and as a consequence it may be easier to identify the molecular events that are critical for the process to take place than in systems such as leaf senescence. The material will be collected from 20 Arabidopsis thaliana ecotype Columbia plants  (NASC order number N1093) grown under controlled conditions specified under NASC protocol: Baseline sample preparation. Two pods will be collected from each plant and pooled from 20 plants to reduce sampling variability. It is intended that 3 replicates of the experiment will be submitted.  The RNA will be extracted from mature green silique tissues of 10 days after anthesis and senescing pod tissues of 20 days after anthesis using lithium chloride extraction protocol and cleaned with Qiagen RNAeasy column. Experimenter name = Thomas Yang; Experimenter phone = 01159516378; Experimenter institute = University of Nottingham; Experimenter address = Division of Plant Sciences; Experimenter address = University of Nottingham; Experimenter address = Sutton Bonington Campus; Experimenter address = Loughborough; Experimenter address = Leicestershire; Experimenter zip/postal_code = LE12 5RD; Experimenter country = UK Experiment Overall Design: 6 samples were used in this experiment				
Protocol Name	Affymetrix:Protocol:Hybridization-Unknown	P-AFFY-6			
Protocol Type	hybridization	feature_extraction			
Protocol Description	Title: Affymetrix Generic Hybridization. Description: 	Title: Affymetrix CEL analysis. Description: 			
Protocol Parameters					
Protocol Hardware					
Protocol Software	MicroArraySuite 5.0	MicroArraySuite 5.0			
Protocol Contact					
Protocol Term Source REF	mo				
SDRF File	E-GEOD-5736.sdrf.txt				
Term Source Name	NCBI Taxonomy	The MGED Ontology	ArrayExpress	EFO	mo
Term Source File	http://www.ncbi.nlm.nih.gov/Taxonomy/	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php
Term Source Version