Comment[ArrayExpressAccession]	E-GEOD-57273
MAGE-TAB Version	1.1					
Public Release Date	2014-05-06
Investigation Title	Gene expression profiles of Liuwei Dihuang Pill treatment postmenopausal osteoporosis with kidney Yin deficiency					
Comment[Submitted Name]	Gene expression profiles of Liuwei Dihuang Pill treatment postmenopausal osteoporosis with kidney Yin deficiency
Experiment Description	Investigate gene expression profiles of Liuwei Dihuang Pill treatment postmenopausal osteoporosis with kidney Yin deficiency in peripheral blood Liuwei Dihuang Pill (LDP), a classic Chinese medicinal formula, has been used to treat PMO with kidney YIN deficiency for three months. Whole human genome oligo microarray were applied to explore the differentially expressed genes before and after LDP treatment.					
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl				
Person Last Name	Xie	Ge	Xie	Li	Chen	Xu
Person First Name	LH	Jirong	Lihua	Shengqiang	Juan	Huijuan
Person Email	geo@ncbi.nlm.nih.gov					
Person Affiliation	Fujian Academy of Traditional Chinese Medicine					
Person Address	Fujian Academy of Traditional Chinese Medicine, 282hao,Wusi Road ,Gulou distract, Fuzhou, China					
Person Roles	submitter					
Protocol Name	P-GSE57273-1	P-GSE57273-3	P-GSE57273-4	P-GSE57273-2	P-GSE57273-5	
Protocol Description	Add the images (.tif) to be extracted to the Agilent Feature Extraction (FE) software Project. Select Project > Start Extracting and export data to txt.Raw signal intensities were normalized in quantile method by GeneSpring GX v11.5.1, and low intensity genes were filtered (Genes that at least 5 out of 15 samples have flags in Detected were chosen for further analysis). ID_REF =  VALUE = To identify differentially expressed genes, we performed a Volcano Plot filtering between the two compared samples from the experiment. The threshold is Fold Change >= 1.5, P-value <= 0.05.	if the yield is <1.65μg and the specific activity is <9.0 pmol Cy3 or Cy5 per μg cRNA do not proceed to the hybridization step. Repeat cRNA preparation.	100 μl of hybridization solution was dispensed into the gasket slide and assembled to the gene expression microarray slide. The slides were incubated for 17 hours at 65°C in an Agilent Hybridization Oven.	Five (5) ml of peripheral blood was taken from each patient. Mononuclear cells were isolated via lymphocyte lysing.And then add 1 ml trizol reagents.	Agilent Microarray Scanner (Agilent p/n G2565BA),Scan region Scan Area (61 x 21.6 mm),Scan resolution:5μm,scanning mode Single Pass.Click Scan Slot m-n on the Scan Control main window where the letter m represents the Start slot where the first slide is located and the letter n represents the End slot where the last slide is located.	
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	nucleic acid extraction protocol	array scanning protocol	
Experimental Factor Name	TIME	AGE				
Experimental Factor Type	time	age				
Comment[SecondaryAccession]	GSE57273					
Comment[GEOReleaseDate]	2014-05-06					
Comment[ArrayExpressSubmissionDate]	2014-05-05					
Comment[GEOLastUpdateDate]	2014-05-06					
Comment[AEExperimentType]	transcription profiling by array					
SDRF File	E-GEOD-57273.sdrf.txt