Comment[ArrayExpressAccession]	E-GEOD-56666
MAGE-TAB Version	1.1					
Public Release Date	2014-04-10
Investigation Title	Genetic Diversity Influences the Response of the Brain to Developmental Lead Exposure.					
Comment[Submitted Name]	Genetic Diversity Influences the Response of the Brain to Developmental Lead Exposure.
Experiment Description	In this study we analyzed the effects of lead-exposure up hippocampal gene expression in males and females exposed to 0ppm, 250ppm and 750ppm lead during two different developmental periods, perinatal (in utero through to weaning at PND21) and postnatal (PND0-PND45), across three strains (Fischer, Long Evans and Sprague Dawley).  All tissue was taken at PND 55.  We used affymetrix Rat Gene 1.0ST arrays to obtain global gene expression data from each animal, with a group size of 4 for all conditions (Total number of Arrays = 119) Gene expression was profiled in the hippocampus of rats at no lead exposure (0ppm), 250ppm and 750 ppm lead exposure levels during perinatal and postnatal developmental periods of both males and females of three strains of rat (Fischer, Long Evans and Sprague Dawley).					
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl				
Person Last Name	Anderson	Schneider	Anderson	Talsania		
Person First Name	David	Jay	David	Keyur		
Person Mid Initials	William					
Person Email	David.Anderson@jefferson.edu					
Person Affiliation	Thomas Jefferson University					
Person Phone	2155038827					
Person Address	Pathology, Anatomy & Cell Biology, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA, USA					
Person Roles	submitter					
Protocol Name	P-GSE56666-1	P-GSE56666-4	P-GSE56666-5	P-GSE56666-2	P-GSE56666-3	P-GSE56666-6
Protocol Description	Raw gene expression data was normalized using RMA in Partek Genomics Suit. ID_REF =  VALUE = RMA normalized signal intensity (no batch correction)	standard Affymetrix protocol	standard Affymetrix protocol	Animals received either perinatal or postnatal exposure to control chow, control chow containing 250ppm lead-acetate or control chow containing 750ppm lead acetate. Perinatal exposure consisted of the dams being placed on lead food for 10 days pior to breeding and remaining on this food until weening, at which time the weaned pups were placed on control food until the end of the experiment at PND55. Postnatal animals only received lead-containgin food from brith through to PND 45, then were placed on control food until PND 55. Animals were euthanized and hippocampii immediately dissected and flash frozen. Samples were then stored at -80 degrees C until processed.	Tissue was extracted using Qiagen miRNeasy kits according to the manufacturers protocol on a Qiagen Qiacube Automated Extraction system. Following extraction samples underwent QA/QC checks using a GE NanoVue Spectrophotometer and were then run on an Agilent Bioanalyzer 2100 for RNA integerity.	Chips were scanned using Affymetrix GeneChip Scanner 3000.
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	TREATMENT	STRAIN	DEVELOPMENTAL PERIOD	SEX		
Experimental Factor Type	treatment	strain	developmental period	sex		
Comment[SecondaryAccession]	GSE56666					
Comment[GEOReleaseDate]	2014-04-10					
Comment[ArrayExpressSubmissionDate]	2014-04-09					
Comment[GEOLastUpdateDate]	2014-04-10					
Comment[AEExperimentType]	transcription profiling by array					
SDRF File	E-GEOD-56666.sdrf.txt