Comment[ArrayExpressAccession]	E-GEOD-55033
MAGE-TAB Version	1.1						
Public Release Date	2015-09-03
Investigation Title	MicroRNA-15/16 Antagonizes c-Myb to Control Natural Killer Cell Maturation						
Comment[Submitted Name]	MicroRNA-15/16 Antagonizes c-Myb to Control Natural Killer Cell Maturation
Experiment Description	NK cells develop in the bone marrow and complete their maturation in peripheral organs, but the molecular events controlling maturation are incompletely understood. Utilizing an NK cell-specific miR-15/16 deficient genetic model (15aKO), we identified a critical role for miR-15/16 family microRNAs in the normal maturation of NK cells in vivo, with a specific reduction in mature CD11b+CD27- NK cells in multiple tissues. The mechanism responsible was a block in differentiation, since accelerated NK cell death was not evident, and earlier intermediates of NK cell maturation were expanded. Further, we identified Myb as a direct target of miR-15/16 in NK cells, with Myb expression increased in immature 15aKO NK cells. Following adoptive transfer, immature 15aKO NK cells exhibited defective maturation, which was rescued by ectopic miR-15/16 expression or Myb knockdown. Moreover, Myb overexpression resulted in defective NK cell maturation. Thus, miR-15/16 regulation of Myb controls the normal NK cell maturation program. 3 technical replicates each of CD27+ 15a/16-1FKO NK cells, and CD27+ Ctrl NK cells						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Fehniger	Sullivan	Fehniger				
Person First Name	Todd	Ryan	Todd				
Person Mid Initials	A	P	A				
Person Email	tfehnige@wustl.edu						
Person Affiliation	Washington University in St. Louis School of Medicine						
Person Address	Internal Medicine / Oncology, Washington University in St. Louis School of Medicine, 660 S. Euclid Ave Campus Box 8007, Saint Louis, MO, USA						
Person Roles	submitter						
Protocol Name	P-GSE55033-1	P-GSE55033-5	P-GSE55033-6	P-GSE55033-2	P-GSE55033-3	P-GSE55033-4	P-GSE55033-7
Protocol Description	Analyzed with Partek Genomics Suite  v6.6, using RMA scaling ID_REF =  VALUE = RMA signal	Standard Affymetrix protocol	Standard Affymetrix protocol	Sorted immediately after spleen harvest.	8-12 week old mice	Cellular material was resuspended in Trizol (Invitrogen) and processed using Directzol columns (Zymo Research) according to the manufacturer's instructions.	Standard Affymetrix protocol
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	genotype						
Experimental Factor Type	genotype						
Publication Title	MicroRNA-15/16 Antagonizes Myb To Control NK Cell Maturation.						
Publication Author List	Sullivan RP, Leong JW, Schneider SE, Ireland AR, Berrien-Elliott MM, Singh A, Schappe T, Jewell BA, Sexl V, Fehniger TA						
PubMed ID	26268657						
Publication DOI	10.4049/jimmunol.1500949						
Comment[SecondaryAccession]	GSE55033						
Comment[GEOReleaseDate]	2015-09-03						
Comment[ArrayExpressSubmissionDate]	2014-02-14						
Comment[GEOLastUpdateDate]	2015-09-03						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-55033.sdrf.txt