Comment[ArrayExpressAccession]	E-GEOD-53899
MAGE-TAB Version	1.1					
Public Release Date	2014-01-11
Investigation Title	Effect of neurotrophin receptor p75 (p75NTR) over-expression on the miRNAome of human umbilical vein endothelial cells					
Comment[Submitted Name]	Effect of neurotrophin receptor p75 (p75NTR) over-expression on the miRNAome of human umbilical vein endothelial cells
Experiment Description	MicroRNAs (miRNAs) are small non-protein-coding RNAs that are incorporated into the RNA-induced silencing complex (RISC) and inhibit gene expression by regulating the stability and/or the translational efficiency of target mRNAs. p75NTR, which is scarcely present in healthy endothelial cells (ECs), becomes strongly expressed by capillary ECs after induction of peripheral ischemia in type-1 diabetic mice. p75NTR expression promotes endothelial cells apoptosis and inhibits angiogenesis. In order to identify miRNAs sub-sequentely modulated by p75NTR, miRNA expression profiles of human umbilical vein endothelial cells (HUVEC) over-expressing p75NTR were generated, allowing the identification of miRNAs modulated upon p75NTR up-regulation. HUVEC over-expressing p75NTR or Null (empty vector) were generated by adenoviral infection. miRNA expression profiles were then measured and miRNAs modulated upon p75NTR up-regulation were identified.					
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl				
Person Last Name	Caporali	Emanueli	Caporali	Voellenkle	Martelli	
Person First Name	Andrea	C	A	C	F	
Person Email	a.caporali@ed.ac.uk					
Person Affiliation	University of Edinburgh					
Person Address	University of Edinburgh, 47 Litlte France Crescent, EDINBURGH, United Kingdom					
Person Roles	submitter					
Protocol Name	P-GSE53899-4	P-GSE53899-5	P-GSE53899-1	P-GSE53899-2	P-GSE53899-3	P-GSE53899-6
Protocol Description	2.5 ug total RNA was labeled with Hy3, Hy5 resepctively according to the manufacturer's protocol (miRCURY LNA microRNA Array Power labeling kit, EXIQON).	2-color hybridization was performed using miRCURY LNA microRNA Arrays (v.10.0, EXIQON). 2.5ug of each labeled RNA were co-hybridized at 53°C for 16 hours. Hybridization and washing steps were performed on the HS 400 PRO hybridization station (Tecan).	To obtain p75 over-expressing cells, HUVEC were infected by adenoviral vectors carrying human p75NTR sequence (Ad. p75NTR) or by empty vector (Ad-rad66).	HUVEC were grown in EGM-2 (Lonza) containing 2% FBS.	Trizol (Invitrogen) extraction of total RNA was performed according to the manufacturer's instructions.	MicroRNA arrays were scanned using the GenePix 4100A microarray scanner and the GenePix Pro 6.0.1.25 software (Molecular Devices).
Protocol Type	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Comment[SecondaryAccession]	GSE53899					
Comment[GEOReleaseDate]	2014-01-11					
Comment[ArrayExpressSubmissionDate]	2014-01-08					
Comment[GEOLastUpdateDate]	2014-01-12					
Comment[AEExperimentType]	transcription profiling by array					
Comment[AdditionalFile:Data1]	GSE53899_Log2FC_p-val.txt					
SDRF File	E-GEOD-53899.sdrf.txt