Comment[ArrayExpressAccession]	E-GEOD-53496
MAGE-TAB Version	1.1						
Public Release Date	2014-04-29
Investigation Title	Expression profiling in control and YY1 knockout mouse intestinal crypt epithelia						
Comment[Submitted Name]	Expression profiling in control and YY1 knockout mouse intestinal crypt epithelia
Experiment Description	Crypts were isolated from either control or YY1f/f; Vil-Cre-ERT2 mice treated with tamoxifen for 4 days to induce knockout Jejunal crypt epithelia were isolated and processed for microarray						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Verzi	Perekatt	Verzi				
Person First Name	Michael	Ansu	Mike				
Person Email	geo@ncbi.nlm.nih.gov						
Person Affiliation	Rutgers, the State University of New Jersey						
Person Address	Genetics, Rutgers, the State University of New Jersey, 145 bevier rd, Piscataway, NJ, USA						
Person Roles	submitter						
Protocol Name	P-GSE53496-1	P-GSE53496-5	P-GSE53496-6	P-GSE53496-2	P-GSE53496-3	P-GSE53496-4	P-GSE53496-7
Protocol Description	data were processed with dChip software ID_REF =  VALUE = dChip signal	total RNA was processed and hybridized according to Affymetrix instructions	Standard Affymetrox protocol.	Crypts were isolated from either control or YY1f/f; Vil-Cre-ERT2 mice treated with tamoxifen for 4 days	mouse jejunum crypt epithelium were isolated using EDTA treatment and filtering through a 70um filter.	RNA was isolated using Trizol	Standard Affymetrox protocol.
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	GENOTYPE						
Experimental Factor Type	genotype						
Comment[SecondaryAccession]	GSE53496						
Comment[GEOReleaseDate]	2014-04-29						
Comment[ArrayExpressSubmissionDate]	2013-12-19						
Comment[GEOLastUpdateDate]	2014-04-30						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-53496.sdrf.txt