Investigation Title	Transcription profiling of mouse epididymis throughout development to identify cell-specific genes that can be used as biomarkers for key differentiation events.	
Comment[Submitted Name]	Gene expression in whole epididymus at gd 12, 14, 16, 18 and pnd2	
Experimental Design	development_or_differentiation_design	co-expression_design	transcription profiling by array	
Experimental Design Term Source REF	mo:1.3.1.1	mo	EFO	
Comment[ArrayExpressReleaseDate]	2007-11-13	
Comment[SecondaryAccession]	GSE5333	
Comment[AEMIAMESCORE]	3	
Comment[SecondaryAccession]	GDS2202	
Comment[ArrayExpressAccession]	E-GEOD-5333	
Comment[MAGETAB TimeStamp_Version]	2010-08-06 16:07:19 Last Changed Rev: 13058	
Experimental Factor Name	OrganismPart	Age	
Experimental Factor Type	organism_part	age	
Experimental Factor Term Source REF	
Person Last Name	Sharghi	
Person First Name	Mehran	
Person Mid Initials	
Person Email	gudmap-db@hgu.mrc.ac.uk	
Person Phone	
Person Fax	
Person Address	MRC Human Genetics Unit, Crewe Road, Edinburgh, EH4 2XU, United Kingdom	
Person Affiliation	MRC Human Genetics Unit	
Person Roles	submitter	
Person Roles Term Source REF		
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Quality Control Term Source REF	
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Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2007-11-13	
PubMed ID	
Publication DOI	
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Publication Title	
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Publication Status Term Source REF	
Experiment Description	The overall objective of this proposal is to map the temporal and spatial dynamics of gene expression in the fetal mouse testis at key developmental timepoints. Urogenital tract malformations are the most common birth defects in males and their incidence together with other male reproductive health concerns such as reduced fertility and testicular cancer are reportedly on the rise in the human population. To better understand the impact of genetic factors and environmental influences on testicular development, it is important to first understand normal gene expression patterns and signaling cascades within the fetal testis during development. The goal of this study is to identify cell-specific genes that can be used as biomarkers for key differentiation events. Experiment Overall Design: Day to day comparison in whole epididymus throughout development with triplicates at each time point.  Each sample is a pair of epididymus from a single animal and each animal is taken from a different dam.	
Protocol Name	P-G5333-1	P-G5333-2	P-G5333-3	P-G5333-5	
Protocol Type	specified_biomaterial_action	specified_biomaterial_action	nucleic_acid_extraction	hybridization	
Protocol Description	Dissect method: Whole organ excision	Dissect method: NA	Gaido protocol: 'RNA isolation from whole testes'. Target Amplified manufacturer/kit: Affymetrix Target Amplified protocol: Affymetrix Two-Cycle cDNA synt Rounds of amplification2	Amount labeled  target hybridization to array: 15 ug	
Protocol Parameters	
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Protocol Contact	
Protocol Term Source REF			The MGED Ontology	
SDRF File	E-GEOD-5333.sdrf.txt	
Term Source Name	EFO	ncbitax	The MGED Ontology	ArrayExpress	mo:1.3.1.1	mo	EFO	The MGED Ontology	
Term Source File	http://www.ebi.ac.uk/efo/	http://www.ncbi.nlm.nih.gov/Taxonomy/taxonomyhome.html	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	
Term Source Version					1.3.1.1