Comment[ArrayExpressAccession]	E-GEOD-52343
MAGE-TAB Version	1.1						
Public Release Date	2014-04-29
Investigation Title	Cdk8, Cyclin C, Med12 or Med13 depletion effect on gene expression in Drosophila S2 cells						
Comment[Submitted Name]	Cdk8, Cyclin C, Med12 or Med13 depletion effect on gene expression in Drosophila S2 cells
Experiment Description	Expression profiling following depletion of Mediator Cdk8 module subunits Cdk8, Cyclin C (CycC), Med12 and Med13 72 hours after dsRNA treatment of Drosophila melanogaster S2 cells. Results provide insight into the role of individual Cdk8 module subunits in regulation of transcription. 22 samples. 2 Cdk8 dsRNA, 4 CycC dsRNA, 4 Med12 dsRNA, 4 Med13 dsRNA, 8 control samples including 4 Luciferase (Luc) dsRNA and 4 GFP dsRNA						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Kuuluvainen	Kuuluvainen	Mäkelä				
Person First Name	Emilia	E	T				
Person Mid Initials			P				
Person Email	emilia.kuuluvainen@helsinki.fi						
Person Affiliation	University of Helsinki						
Person Address	Institute of Biotechnology, University of Helsinki, Viikinkaari 9, Helsinki, Finland						
Person Roles	submitter						
Protocol Name	P-GSE52343-1	P-GSE52343-5	P-GSE52343-6	P-GSE52343-2	P-GSE52343-3	P-GSE52343-4	P-GSE52343-7
Protocol Description	GeneSpring GX 12.1 GC-RMA ID_REF =  VALUE = GC-RMA normalized	Biotinylated cRNA was prepared from 4µg total RNA according to standard Affymetrix protocol (Expression Analysis Technical Manual P/N 702232 Rev.2)	10 µg of fragmented cRNA were hybridized on GeneChip Drosophila Genome 2.0 arrays according to Affymetrix standars protocols (Expression Analysis Technical Manual P/N 702232 Rev.2). Affymetrix Fluidics station 450 was used for washing and staining.	1.2 million exponentially growing S2 cells were treated with 20µg dsRNA in 1 ml of serum free media on 6-well plates. Serum containing media was added 1h later for a final FBS concentration of 10%. Cells were cultured for 72h before sample collection.	Drosophila S2 cells were cultured in Drosophila SFM media (Gibco) supplemented with 10% FBS, L-glutamine and antibiotics	Total RNA, Qiagen RNeasy Plus Mini Kit according to manufcturers protocol	GeneChips were scanned with Affymetrix 3000 scanner
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	TREATED WITH						
Experimental Factor Type	treated with						
Comment[SecondaryAccession]	GSE52343						
Comment[GEOReleaseDate]	2014-04-29						
Comment[ArrayExpressSubmissionDate]	2013-11-13						
Comment[GEOLastUpdateDate]	2014-04-29						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-52343.sdrf.txt