Comment[ArrayExpressAccession]	E-GEOD-50867
MAGE-TAB Version	1.1					
Public Release Date	2013-09-14
Investigation Title	Identification of microRNA as novel circulating markers for presence of intracranial aneurysms					
Comment[Submitted Name]	Identification of microRNA as novel circulating markers for presence of intracranial aneurysms
Experiment Description	We carried out a case control study in an attempt to identify changes in circulating microRNAs in patients with intracranial aneurysms (IAs). We selected 40 cases (20 ruptured and 20 unruptured) and 20 healthy controls. We randomly selected 5 samples from each group and combined them into a sample pool. In this way we obtained 12 sample pools and one pool was used for a single microarray. Changes in microRNA levels in the plasma were surveyed with Agilent Human microRNA Microarray (Release 14.0, 8x15K). We identified 20 microRNAs that were unanimously changed in both ruptured and unruptured patients. We included 40 cases (20 ruptured and 20 unruptured) and 20 healthy controls. We randomly selected 5 plasma samples from each group and combined them into a sample pool. In this way we obtained 12 sample pools and one pool was used for a single microarray. Total RNA was isolated from 1 ml plasma from each sample pool and resuspended in the same volume of buffer. A fixed volume of RNA sample was used for microarray detection.					
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl				
Person Last Name	Jiang	Li	Zhang	Wu	Yang	Jiang
Person First Name	Fan	Peng-Xiang	Qunye	Xiao	Xinjian	Fan
Person Email	fjiang@sdu.edu.cn					
Person Affiliation	Shandong University					
Person Phone	+86 531 8216 9267					
Person Address	Shandong University, 107 Wen Hua Xi Road, Jinan, Shandong, China					
Person Roles	submitter					
Protocol Name	P-GSE50867-1	P-GSE50867-3	P-GSE50867-4	P-GSE50867-2	P-GSE50867-5	
Protocol Description	Raw data were normalized by Quantile algorithm, Gene Spring Software 11.0(Agilent technologies, Santa Clara, CA, US). ID_REF =  VALUE = Normalized signal intensity FLAGS = 	miRNA was labeled by miRNA Complete Labeling and Hyb Kitn(Cat#5190-0456, Agilent technologies, Santa Clara, CA, US) following the manufacturer’s instruction's labeling section.	Each slide was hybridized with 100ng Cy3-labeled RNA using miRNA Complete Labeling and Hyb Kit(Cat#5190-0456, Agilent technologies, Santa Clara, CA, US)in hybridization Oven(Cat#G2545A, Agilent technologies, Santa Clara, CA, US)at 55℃，20rpm for 20 hours according to the manufacturer’s instructions, hybridization section. After hybridization, slides were washed in staining dishes (Cat#121, Thermo Shandon, Waltham, MA, US) with Gene Expression Wash Buffer Kit(Cat#5188-5327, Agilent technologies, Santa Clara, CA, US).	Total RNA was extracted and purified using mirVana™ miRNA Isolation Kit (Cat#AM1560, Ambion, Austin, TX, US) following the manufacturer’s instructions and checked by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US)	Slides were scanned by Agilent Microarray Scanner(Cat#G2565BA，Agilent technologies, Santa Clara, CA, US)and Feature Extraction software 10.7 (Agilent technologies, Santa Clara, CA, US)with default settings.	
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	nucleic acid extraction protocol	array scanning protocol	
Comment[SecondaryAccession]	GSE50867					
Comment[GEOReleaseDate]	2013-09-14					
Comment[ArrayExpressSubmissionDate]	2013-09-13					
Comment[GEOLastUpdateDate]	2013-09-15					
Comment[AEExperimentType]	transcription profiling by array					
SDRF File	E-GEOD-50867.sdrf.txt