Comment[ArrayExpressAccession]	E-GEOD-50002
MAGE-TAB Version	1.1					
Public Release Date	2013-08-20
Investigation Title	Effect of Twist-box mutation on gene expression induced by Twist1 overexpression					
Comment[Submitted Name]	Effect of Twist-box mutation on gene expression induced by Twist1 overexpression
Experiment Description	Twist1 is a transcription factor that induces EMT and drives metastasis in prostate cancer. We examined global gene expression in Myc-CaP mouse prostate cancer cells following overexpression of Twist1 and the Twist1 mutants F191G, AQA, and DQD. 15 total samples were analysed, with 3 replicates of 5 groups: Twist1-WT, Twist1-AQA, Twist1-DQD, Twist1-F191G, and vector control. We performed the comparisons: WT > VEC, F191G > VEC. Samples were normalized by Twist1 expression in addition to normal					
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl				
Person Last Name	Williams	Gajula	Tran			
Person First Name	Russell	R	P			
Person Email	russell.d.williams@gmail.com					
Person Affiliation	Johns Hopkins University					
Person Address	Radiation Oncology, Johns Hopkins University, 1550 Orleans St, Baltimore, MD, USA					
Person Roles	submitter					
Protocol Name	P-GSE50002-1	P-GSE50002-4	P-GSE50002-5	P-GSE50002-2	P-GSE50002-3	P-GSE50002-6
Protocol Description	All analysis performed in R. Data normalized using RMA from the limma package, and further normalization for Twist1-expression was carried out. For R script used for analysis, see supplemental data (Gajula et al., 2013) ID_REF =  VALUE = RMA normalized signal intensity.	Affymetrix WT Labeling kit, according to manufacturer's instructions	Affymetrix Mouse Exon 1.0 arrays, according to manufacturer's instructions, washed with Fluidics 450	after transfection and selection, cells were grown to confluence and harvested	Trizol RNA purification	Affymetrix M10 scanner, using software Affymetrix Genechip Command Console v2.0.0.1029
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	BATCH	KNOCK-IN				
Experimental Factor Type	batch	knock-in				
Comment[SecondaryAccession]	GSE50002					
Comment[GEOReleaseDate]	2013-08-20					
Comment[ArrayExpressSubmissionDate]	2013-08-19					
Comment[GEOLastUpdateDate]	2013-08-21					
Comment[AEExperimentType]	transcription profiling by array					
SDRF File	E-GEOD-50002.sdrf.txt