Comment[ArrayExpressAccession]	E-GEOD-49855
MAGE-TAB Version	1.1														
Public Release Date	2013-08-14
Investigation Title	HuR and miR-1192 regulate myogenesis by modulating the translation of HMGB1 mRNA (miRNA)														
Comment[Submitted Name]	HuR and miR-1192 regulate myogenesis by modulating the translation of HMGB1 mRNA (miRNA)
Experiment Description	Upon muscle injury the high mobility group box 1 (HMGB1) protein is up-regulated and secreted to initiate reparative responses. Here we show that HMGB1 controls myogenesis both in vitro and in vivo, during development and after adult muscle injury. HMGB1 expression in muscle cells is regulated at the translational level: the miRNA miR-1192 inhibits HMGB1 translation and the RNA-binding protein HuR promotes it. HuR binds to a cis-element, HuRBS, located in the 3'UTR of the HMGB1 transcript, and at the same time miR-1192 is recruited to an adjacent seed element. The binding of HuR to the HuRBS prevents the recruitment of Argonaute 2 (Ago2), overriding miR-1192-mediated translation inhibition.  Depleting HuR reduces myoblast fusion and silencing miR-1192 re-establishes the fusion potential of HuR-depleted cells. We propose that HuR promotes the commitment of myoblasts to myogenesis by enhancing the translation of HMGB1 and suppressing the translation inhibition mediated by miR-1192. RNA content was extracted following immunoprecipitation of HuR using a monoclonal antibody (3A2) and the levels of mRNA were compared to an IgG control in order to determine which transcripts were enriched in the HuR ribonucleoprotein complex.														
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl													
Person Last Name	Gallouzi	Dormoy-Raclet	Cammas	Celona	Lian	van der Giessen	Zivojnovic	Brunelli	Riuzzi	Sorci	Wilhelm	Di Marco	Donato	Bianchi	Gallouzi
Person First Name	Imed	Virginie	Anne	Barbara	Xian	Kate	Marija	Silvia	Francesca	Guglielmo	Brain	Sergio	Rosario	Marco	Imed
Person Mid Initials											T			E	
Person Email	imed.gallouzi@mcgill.ca														
Person Affiliation	McGill University														
Person Phone	514-398-4537														
Person Address	Biochemistry, McGill University, 3655 Promenade Sir William Osler, Montreal, QC, Canada														
Person Roles	submitter														
Protocol Name	P-GSE49855-1	P-GSE49855-5	P-GSE49855-6	P-GSE49855-2	P-GSE49855-3	P-GSE49855-4	P-GSE49855-7								
Protocol Description	Array Pro software was used to calculate differences in signal intensities and to normalize by Z-score transformation ID_REF =  VALUE = Averaged z data	RNA was reverse transcribed in the presence of [α-33P]dCTP.	RNA was denatured with NaOH and spotted onto S&S Nytran SuperCharge nylon membranes. Twenty-four 384-well plates were printed using a BioRobotics MicroGrid Arrayer.	No treatment	Proliferating C2C12 muscle cells were grown in DMEM supplemented with 20% FBS and 1% Pen/Strep. At 80% confluency, cells were collected.	RNA was extracted with phenol/chloroform from C2C12 cell extracts that were subject to immunoprecipitation with an antibody that recognized either HuR or IgG.	the submiitter has not supplied the information								
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol								
Experimental Factor Name	ANTIBODY														
Experimental Factor Type	antibody														
Comment[SecondaryAccession]	GSE49855														
Comment[GEOReleaseDate]	2013-08-14														
Comment[ArrayExpressSubmissionDate]	2013-08-13														
Comment[GEOLastUpdateDate]	2013-08-14														
Comment[AEExperimentType]	transcription profiling by array														
Comment[AdditionalFile:Data1]	GSE49855_raw_and_normalized.txt														
SDRF File	E-GEOD-49855.sdrf.txt