Comment[ArrayExpressAccession]	E-GEOD-49235
MAGE-TAB Version	1.1						
Public Release Date	2014-08-31
Investigation Title	Real-time quantitative PCR analysis of microRNA expression in neonatal and adult cardiovascular progenitors						
Comment[Submitted Name]	Real-time quantitative PCR analysis of microRNA expression in neonatal and adult cardiovascular progenitors
Experiment Description	Human cardiovascular stem cells were isolated from adult (57-75 year old) and neonatal (<1 month old) atrial tissue.  Cardiovascular stem cells were then cloned by single cell dilution and compared using sabiosciences cell development & differentiation miRNA PCR array. microRNA expression profiling by RT-PCR,  3 cardiac progenitor cell clones isolated from adults were run separately and results were pooled and compared to 8 neonatal cardiovascular progenitor cell clones that were run separately and pooled.						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Kearns-Jonker	Fuentes	Kearns-Jonker				
Person First Name	Mary	Tania	Mary				
Person Mid Initials		I					
Person Email	geo@ncbi.nlm.nih.gov						
Person Affiliation	Loma Linda University						
Person Address	Anatomy, Loma Linda University, 25094 Daisy Ave, Loma Linda, CA, USA						
Person Roles	submitter						
Protocol Name	P-GSE49235-1	P-GSE49235-5	P-GSE49235-6	P-GSE49235-2	P-GSE49235-3	P-GSE49235-4	P-GSE49235-7
Protocol Description	Data normalization and analysis was performed according to the manufacturers instructions using their web-based software package: http://sabiosciences.com/pcrarraydataanalysis.php For normalization the average of 4 housekeeping genes (SNORD48, SNORD47, SNORD44, RNUG-2) was used. The sabiosciences web-based software performs all statistical analysis and deltadeltaCt based fold-change calculations. Fold change worksheet reports test/control  (i.e. Cardiovascular progenitor cells/human embryonic stem cell) and test/test  (i.e. adult cardiovascular progenitor cell clones/neonatal cardiovascular progenitor cell clones) ratios. ID_REF =  VALUE = Matrix normalized worksheet report signal against an average of all houskeeping genes	PCR arrays were performed using the cell development & differentiation miRNA PCR array (SA Biosciences, Frederick, MD) following the Manufacturer's instructions. Reverse transcription was performed using 200ng of total RNA with the RT2 miRNA First Strand Kit (SA Biosciences). Quantitative real-time PCR were performed (IQ5, Bio-rad) with 40 cycles at 94oC for 15 seconds, 60oC for 60 seconds.	n/a	n/a	Cells were grown in M199 media (Invitrogen, Grand Island, NY) supplemented with EGM-2 media (Invitrogen) at 5% CO2.	Total RNA was extracted using Trizol Reagent Protocol.	n/a
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	SAMPLE TYPE						
Experimental Factor Type	sample type						
Comment[SecondaryAccession]	GSE49235						
Comment[GEOReleaseDate]	2014-08-31						
Comment[ArrayExpressSubmissionDate]	2013-07-25						
Comment[GEOLastUpdateDate]	2014-08-31						
Comment[AEExperimentType]	transcription profiling by RT-PCR						
Comment[AdditionalFile:Data1]	GSE49235_fold-change.txt						
Comment[AdditionalFile:Data2]	GSE49235_non-normalized.txt						
SDRF File	E-GEOD-49235.sdrf.txt