Comment[ArrayExpressAccession] E-GEOD-49076 MAGE-TAB Version 1.1 Public Release Date 2013-07-23 Investigation Title Transcriptional profiling of Salmonella typhimurium in the ceca of gnotobiotic mice Comment[Submitted Name] Transcriptional profiling of Salmonella typhimurium in the ceca of gnotobiotic mice Experiment Description Transcriptional profiling of Salmonella typhimurium in the ceca of germ-free and Bacteroides thetaiotaomicron-monoassociated gnotobiotic mice. Comparison with response in MM-Glucose. In vivo transcriptional profiles of Salmonella typhimurium obtained from biological triplicate samples taken from cecal contents of germ-free and Bacteroides thetaiotaomicron-monoassociated mice 5 days post-infection with S. typhimurium. Biological duplicate samples from in vitro culture of S. typhimurium in MM-Glucose used for comparision. Term Source Name ArrayExpress EFO Term Source File http://www.ebi.ac.uk/arrayexpress/ http://www.ebi.ac.uk/efo/efo.owl Person Last Name Sonnenburg Ng Sonnenburg Person First Name Justin K J Person Mid Initials M L Person Email jsonnenburg@stanford.edu Person Affiliation Stanford University Person Phone 650-721-1510 Person Address Microbiology and Immunology, Stanford University, 299 Campus Drive, Stanford, CA, USA Person Roles submitter Protocol Name P-GSE49076-1 P-GSE49076-3 P-GSE49076-4 P-GSE49076-2 P-GSE49076-5 Protocol Description The raw data were RMA-MS normalized using R-2.9.1. and log2 transformed, excluding other species present on chip. ID_REF = VALUE = log2 RMA normalized signal Enzo Biochemicals Bioarray terminal labeling kit standard Affymetrix protocol Phenol-chloroform with bead-beating, Qiagen Rneasy mini column extraction, Ambion DNA free treatment standard Affymetrix protocol Protocol Type normalization data transformation protocol labelling protocol hybridization protocol nucleic acid extraction protocol array scanning protocol Experimental Factor Name TREATMENT Experimental Factor Type treatment Comment[SecondaryAccession] GSE49076 Comment[GEOReleaseDate] 2013-07-23 Comment[ArrayExpressSubmissionDate] 2013-07-22 Comment[GEOLastUpdateDate] 2013-07-24 Comment[AEExperimentType] transcription profiling by array SDRF File E-GEOD-49076.sdrf.txt