Comment[ArrayExpressAccession]	E-GEOD-48997						
MAGE-TAB Version	1.1						
Public Release Date	2013-07-20						
Investigation Title	Expression data in wt or mutant Drosophila melanogaster embryos						
Comment[Submitted Name]	Expression data in wt or mutant Drosophila melanogaster embryos						
Experiment Description	Terminal differentiation of epidermal cells in Drosophila embryos requires the activity of a transcription factor. Svb is necessary and sufficient to induce this process. pri is a regulator of Svb activity, converting it from a repressor into an activator. To characterize the downstream Svb and pri effectors in cell morphogenesis, we performed microarrays in wt, svb -/- (no gene) and pri -/- (svb repressor) mutant conditions. Embryos were selected at a precise 13-15h (after egg laying) stage of development, and manually genotyped for each condition: wt (W samples), svb -/- (R samples) and pri -/- (P samples). 5 independent replicates of 200 embryos are used for each condition. RNA were extracted and hybridized on Affymetrix microarrays.						
Term Source Name	ArrayExpress	EFO					
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	plaza	Menoret					
Person First Name	serge	Delphine					
Person Email	serge.plaza@univ-tlse3.fr						
Person Affiliation	toulouseII University						
Person Address	Developmental Biology Center, toulouseII University, 118 route de Narbonne, Toulouse, France						
Person Roles	submitter						
Protocol Name	P-GSE48997-1	P-GSE48997-5	P-GSE48997-6	P-GSE48997-2	P-GSE48997-3	P-GSE48997-4	P-GSE48997-7
Protocol Description	Data were analyzed with the Microarray Suite version 5.0 (MAS5.0) algorithm using Affymetrix Expression Console software version 1.1. ID_REF =  VALUE = MAS5.0 signal intensity Detection =  the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M) Detection p-value = p-value that indicates the significance level of the detection call	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Data Analysis Manual, 2003, Affymetrix).	Hybridizations were performed as recommended by the manufacturer's instruction for GeneChip Drosophila 2.0 Array.	Embryos were dechorionated with 50% bleach and further genotyped using GFP balancer for homozygote embryos. Selected embryos were rinsed with PBS 0.1% Triton and placed in Trizol solution.	wt, svb +/- or pri +/- flies developed in 2h window at 25 degreeC and aged at 18 degreeC for 13-15h stage.	Total RNA extraction was performed according to the instructions given by the manufacturer of Trizol.	Chips were scanned with the GeneArray Scanner 3000.
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	genotype						
Experimental Factor Type	genotype						
Comment[SecondaryAccession]	GSE48997						
Comment[GEOReleaseDate]	2013-07-20						
Comment[ArrayExpressSubmissionDate]	2013-07-17						
Comment[GEOLastUpdateDate]	2013-07-21						
Comment[AEExperimentType]	transcription profiling by array						
Comment[AdditionalFile:Data1]	GSE48997_differentially_expressed.txt						
SDRF File	E-GEOD-48997.sdrf.txt