Comment[ArrayExpressAccession]	E-GEOD-48191					
MAGE-TAB Version	1.1					
Public Release Date	2013-06-22					
Investigation Title	The gene expression profiles of WT and NML-KO livers					
Comment[Submitted Name]	The gene expression profiles of WT and NML-KO livers					
Experiment Description	To examine whether energy starvation caused by the increase in rRNA transcription affects liver metabolism, we compared the gene expression profiles of WT and NML-KO livers using Affymetrix microarray technology. We analyzed 5 livers of WT mice and 5 livers of NML-KO mice.					
Term Source Name	ArrayExpress	EFO				
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl				
Person Last Name	Oie	Iwasaki	Oie			
Person First Name	Shohei	Naoya	Shohei			
Person Email	geo@ncbi.nlm.nih.gov					
Person Affiliation	Tsukuba university					
Person Address	Tsukuba university, 1-1-1 tennodai, 305-8577, Japan					
Person Roles	submitter					
Protocol Name	P-GSE48191-1	P-GSE48191-4	P-GSE48191-5	P-GSE48191-2	P-GSE48191-3	P-GSE48191-6
Protocol Description	Data were processed using GeneSpring software. Background correction were applied to probe intensities, gene level expression were summarized from the corrected intensities and then quantile normalized. probe group file: MoEx-1_0-st-v1.r2.pgf meta-probeset file: MoEx-1_0-st-v1.r2.dt1.mm8.mps ID_REF =  VALUE = Quantile normalized gene level expression values from GeneSpring	Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)	Samples were hybridized using Affymetrix hybridization kit materials • Heat cocktails at 99° for 5 minutes, then 42° for 5 minutes centrifuge at 15,000 rpm for 1 minute. • Transfer 80μl of hyb solution to each array, then tape holes. • Hybridize 17 hours at 45° at 60rpm	Mouse livers dissected from 12 weeks age of WT and NML-KO mice	Liver tissues were homogenized in 1 ml of Sepazol and total RNA was extracted according to the manufacturer’s instructions (Nacalai tesque).	Affymetrix Gene ChIP Scanner 3000 7G
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	genotype					
Experimental Factor Type	genotype					
Comment[SecondaryAccession]	GSE48191					
Comment[GEOReleaseDate]	2013-06-22					
Comment[ArrayExpressSubmissionDate]	2013-06-21					
Comment[GEOLastUpdateDate]	2013-06-23					
Comment[AEExperimentType]	transcription profiling by array					
SDRF File	E-GEOD-48191.sdrf.txt					
Publication Title	Hepatic rRNA transcription regulates high-fat-diet-induced obesity.					
Publication Author List	Oie S, Matsuzaki K, Yokoyama W, Tokunaga S, Waku T, Han SI, Iwasaki N, Mikogai A, Yasuzawa-Tanaka K, Kishimoto H, Hiyoshi H, Nakajima Y, Araki T, Kimura K, Yanagisawa J, Murayama A					
PubMed ID	24746822					
Publication DOI	1016/j.celrep.2014.03.038. Epub 2014 Apr 18