Comment[ArrayExpressAccession] E-GEOD-47729 MAGE-TAB Version 1.1 Public Release Date 2013-06-08 Investigation Title Global peripheral blood gene expression study and trans-eQTLs Comment[Submitted Name] Global peripheral blood gene expression study and trans-eQTLs Experiment Description This SuperSeries is composed of the SubSeries listed below. Refer to individual Series Term Source Name ArrayExpress EFO Term Source File http://www.ebi.ac.uk/arrayexpress/ http://www.ebi.ac.uk/efo/efo.owl Person Last Name Enquobahrie Christiansen Enquobahrie Psaty Person First Name Daniel Mark Daniel Bruce Person Mid Initials A W A M Person Email geo@ncbi.nlm.nih.gov Person Affiliation University of Washington Person Address Cardiovascular Health Research Unit, University of Washington, 1730 Minor Avenue, Seattle, Washington, USA Person Roles submitter Protocol Name P-GSE47729-1 P-GSE47729-2 P-GSE47729-4 P-GSE47729-7 P-GSE47729-5 P-GSE47729-3 P-GSE47729-6 Protocol Description The data were normalized using quantile normalisation (R-2.15.2 RStudio 0.97.36 Bioconductor affy & aroma.light packages). The probes that were not measured across all samples were excluded. ID_REF = VALUE = quantile normalized The data were normalized using quantile normalisation (R-2.15.2 RStudio 0.97.36 Bioconductor affy & aroma.light packages). The probes that were not measured across all samples were excluded. ID_REF = VALUE = quantile normalized Total RNA labeled with biotin using IlluminaM-. TotalPrepM-bM-^DM-"-96 RNA Amplification Kit (Life Technologies Corp., Carlsbad, CA) Total RNA was hybridized onto Illumina HumanHT-12v4 Beadchip arrays (Illumina, San Diego, CA) Total RNA was hybridized onto Illumina HumanHT-12v3 Beadchip arrays (Illumina, San Diego, CA) Total RNA was extracted using PAXgene Blood RNA Kit (QIAGEN Inc., Valencia, CA) Scanned images of the array chips were imported into IlluminaM-bM-^@M-^Ys GenomeStudio Gene Expression Module Protocol Type normalization data transformation protocol normalization data transformation protocol labelling protocol hybridization protocol hybridization protocol nucleic acid extraction protocol array scanning protocol Experimental Factor Name AGE YRS AGE SEX Experimental Factor Type age yrs age sex Comment[SecondaryAccession] GSE47729 Comment[GEOReleaseDate] 2013-06-08 Comment[ArrayExpressSubmissionDate] 2013-06-07 Comment[GEOLastUpdateDate] 2013-06-09 Comment[AEExperimentType] transcription profiling by array SDRF File E-GEOD-47729.sdrf.txt