Comment[ArrayExpressAccession]	E-GEOD-47712
MAGE-TAB Version	1.1					
Public Release Date	2013-06-07
Investigation Title	Functional studies of the Yeast Mediator Tail Module Subunits					
Comment[Submitted Name]	Functional studies of the Yeast Mediator Tail Module Subunits
Experiment Description	The yeast Mediator complex can be divided into three modules, designated Head, Middle and Tail. Tail comprises the Med2, Med3, Med5, Med15 and Med16 protein subunits, which are all encoded by genes that are individually non-essential for viability. In cells lacking Med16, Tail is displaced from Head and Middle. However, inactivation of MED5/MED15 and MED15/MED16 are synthetically lethal, indicating that Tail performs essential functions as a separate complex even when it is not bound to Middle and Head. We have used the N-Degron method to create temperature sensitive (ts) mutants in the Mediator tail subunits Med5, Med15 and Med16 to study the immediate effects on global gene expression when each subunit is individually inactivated, and when MED5/15 or MED15/16 are inactivated together. All Degron constructs were expressed from their normal chromosomal location under the control of their respective endogenous promoters. We isolated RNA from each strain as early as 45 minutes after changing from the permissive to the restrictive growth conditions to minimize possible secondary effects on gene expression that are not directly caused by the Degron construct(s).					
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl				
Person Last Name	Selth	Larsson	Uvell	Selth	Bjorklund	
Person First Name	Luke	Miriam	Hanna	Luke	Stefan	
Person Email	luke.selth@adelaide.edu.au					
Person Affiliation	University of Adelaide					
Person Address	University of Adelaide, Frome Rd, Adelaide, Australia					
Person Roles	submitter					
Protocol Name	P-GSE47712-1	P-GSE47712-4	P-GSE47712-5	P-GSE47712-2	P-GSE47712-3	P-GSE47712-6
Protocol Description	The data were analyzed with robust multi-array average (RMA) algorithm using default settings. ID_REF =  VALUE = RMA signal intensity	One-Cycle Target Labeling kit (Affymetrix)	Following fragmentation, 15 ug of cRNA were hybridized for 16 hr on Affymetrix Yeast Genome 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.	All strains were grown in YPRCu at 24C to approximately 1x10 7 cells/ml. The media was switched to YPGalactose and growth was continued at 24C for 35 minutes. The cells were transferred to pre-warmed YPGalactose (37C) and growth was continued at 37C for 45 minutes before harvesting	RNeasy Mini kit (Qiagen), with on-column Dnase digestion	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	STRAIN OR LINE					
Experimental Factor Type	strain or line					
Comment[SecondaryAccession]	GSE47712					
Comment[GEOReleaseDate]	2013-06-07					
Comment[ArrayExpressSubmissionDate]	2013-06-06					
Comment[GEOLastUpdateDate]	2013-06-08					
Comment[AEExperimentType]	transcription profiling by array					
SDRF File	E-GEOD-47712.sdrf.txt