Comment[ArrayExpressAccession]	E-GEOD-46279
MAGE-TAB Version	1.1						
Public Release Date	2013-06-01
Investigation Title	Expression data from HUVEC adenovirally overexpressing MEF2C						
Comment[Submitted Name]	Expression data from HUVEC adenovirally overexpressing MEF2C
Experiment Description	The transcription factor MEF2C is specifically induced by VEGF in endothelial cells. To delineate target genes of MEF2C in endothelial cells, which might be important during angiogenesis also, MEF2C was overexpressed adenovirally in human umbilical vein endothelial cells (HUVECs) over a period of 8 to 32 hours. Expression data should be compared to control infected cells, to discriminate against virally induced genes, and should be further compared to HUVECs infected with an adenovirus encoding for a dominant-negative form of MEF2. HUVECs of the same batch, passage 3 were infected with Ad.con, Ad.MEF2C or Ad.DNMEF2 for 8, 16 or 32 hours for RNA extraction and hybridization on Affymetrix microarrays.						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Sturtzel	Sturtzel	Bilban				
Person First Name	Caterina	Caterina	Martin				
Person Email	caterina.sturtzel@meduniwien.ac.at						
Person Affiliation	Medical Univ Vienna						
Person Address	Dep f Vasc Bio, Medical Univ Vienna, Lazarettgasse 19, Vienna, Austria						
Person Roles	submitter						
Protocol Name	P-GSE46279-1	P-GSE46279-5	P-GSE46279-6	P-GSE46279-2	P-GSE46279-3	P-GSE46279-4	P-GSE46279-7
Protocol Description	RMA (Robust Multiarray average) Signal extraction using Affymetrix Expression Console ID_REF =  VALUE = RMA signal intensity on base of log2	100ng of total RNA were amplified and labeled with biotin using the Whole Transcript (WT) Sense Target Labeling Protocol.	Affymetrix GeneChip® Human Gene 1.0 ST arrays were washed, stained and scanned according to the protocol described in WT Sense Target Labeling Assay Manual (Fluidics Protocol FS450_0007).	HUVEC received fresh medium and were infected with adenovirus of MOI 10 for indicated time points, cells were harvested in Trizol.	Isolated HUVEC, passage 3, were grown in 6 wells to subconfluent density.	Trizol extraction of total RNA was performed according to the manufacturer's instructions.	Scanning was done on an Affymetrix GeneChip Scanner 3000 7G
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	TREATMENT	TREATMENT DURATION					
Experimental Factor Type	treatment	treatment duration					
Comment[SecondaryAccession]	GSE46279						
Comment[GEOReleaseDate]	2013-06-01						
Comment[ArrayExpressSubmissionDate]	2013-04-22						
Comment[GEOLastUpdateDate]	2013-06-05						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-46279.sdrf.txt