Comment[ArrayExpressAccession]	E-GEOD-45744
MAGE-TAB Version	1.1						
Public Release Date	2013-04-04
Investigation Title	Whole-genome expression data from normal FVB mouse lung tissue, transgenic cyclin E overexpressing (CEO) normal mouse lung tissue, and transgenic CEO lung adenocarcinomas						
Comment[Submitted Name]	Whole-genome expression data from normal FVB mouse lung tissue, transgenic cyclin E overexpressing (CEO) normal mouse lung tissue, and transgenic CEO lung adenocarcinomas
Experiment Description	FVB mice were engineered to express wild-type human cyclin E under control of the human surfactant C promoter (CEO mice; Ma et al, PNAS 2007).  These mice develop spontaneous lung tumors, which were shown to be adenocarcinoma by histological analysis.  Here we compare whole-genome RNA expression levels between the tumors and normal lung of 4 CEO mice as well as 4 nontransgenic animals. RNA was isolated from the lungs of 4 FVB mice and adjacent normal and tumor tissue from 4 FVB transgenes harboring human surfactant protein C- driven wild-type human cyclin E, all 7-11 months in age.  These samples were divided into 3 groups of four, and  12 independent hybridizations were performed for analysis with Affymetrix GeneChip Mouse 430 2.0 arrays.						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Freemantle	Busch	Freemantle				
Person First Name	Sarah	Alexander	Sarah				
Person Mid Initials		M	J				
Person Email	freemantle@dartmouth.edu						
Person Affiliation	Geisel School of Medicine at Dartmouth						
Person Phone	6036501714						
Person Fax	6036501129						
Person Address	Pharmacology and Toxicology, Geisel School of Medicine at Dartmouth, HB7650, Hanover, NH, USA						
Person Roles	submitter						
Protocol Name	P-GSE45744-1	P-GSE45744-5	P-GSE45744-6	P-GSE45744-2	P-GSE45744-3	P-GSE45744-4	P-GSE45744-7
Protocol Description	Raw data was converted to GC-RMA in the GeneSifter Analysis Edition (Geospiza Inc.) for subsequent analysis ID_REF =  VALUE = log2 GC-RMA signal intensity	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA	Hybridizations were performed according to Affymetrix guidelines at the Dartmouth College Microarray Shared Resource using an Affymetrix GeneChip Workstation.	Normal lung tissue and adenocarcinomas were identified by gross pathology and isolated and stored in RNAlater (Qiagen)	FVB nontransgenic and human surfactant protein C promoter-driven human cyclin E expressing transgenes were allowed to age to 7-11 months	Total RNA was extracted with Trizol as per the manufacturer's instructions	GeneChips were scanned at the Dartmouth College Microarray Shared Resource using an Affymetrix GeneChip Workstation
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	STRAIN OR LINE	ORGANISM PART					
Experimental Factor Type	strain or line	organism part					
Comment[SecondaryAccession]	GSE45744						
Comment[GEOReleaseDate]	2013-04-04						
Comment[ArrayExpressSubmissionDate]	2013-04-03						
Comment[GEOLastUpdateDate]	2013-04-16						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-45744.sdrf.txt