Comment[ArrayExpressAccession] E-GEOD-45692 MAGE-TAB Version 1.1 Public Release Date 2013-04-03 Investigation Title The global gene expression pattern by KDX1 over-expression Comment[Submitted Name] The global gene expression pattern by KDX1 over-expression Experiment Description We did transcription profiling on the effect of KDX1 over-expression. Analysis was performed with wild type and KDX1 over-expressed cells ( pRS426-KDX1). Yeast cells were grown on SD-ura medium. Yeast cells were grown overnight at 30M-BM-0C . The culture was refreshed to 0.2 O.D and grown at 30M-BM-0C for 5h 30min. Cells were collected and processed for RNA extraction. Term Source Name ArrayExpress EFO Term Source File http://www.ebi.ac.uk/arrayexpress/ http://www.ebi.ac.uk/efo/efo.owl Person Last Name Chang Chang Yun Person First Name miwha Miwha Cheol-won Person Email teddy05@korea.ac.kr Person Affiliation Korea university Person Phone 82-10-9080-1256 Person Address School of Life Sciences and Biotechnology, Korea university, anam dong 5ga, Seoul, South Korea Person Roles submitter Protocol Name P-GSE45692-1 P-GSE45692-6 P-GSE45692-3 P-GSE45692-8 P-GSE45692-7 P-GSE45692-2 P-GSE45692-4 P-GSE45692-5 Protocol Description ID_REF = VALUE = log ratio KDX1/control Labeled cDNAs were mixed with hybridization solution (MYcroarray.com), and the hybridization mixtures were heated at 65M-bM-^DM-^C for 5 min and then immediately cooled on ice for 5 min. Hybridization mixtures were directly loaded onto assembled MYcroarray.com microarray. The arrays hybridized at 50M-bM-^DM-^C for 16 hr using Agilent Hybridization oven (Agilent Technology, USA). Yeast cell was grown on SD-ura medium. Yeast cell was grown overnight at 30M-0C . The culture was refreshed to 0.2 O.D and grown at 30M-0C for 5h 30min. Cells were collected. The average fluorescence intensity for each spot was calculated and local background was subtracted. All data normalization and selection of fold-changed genes were performed using GeneSpring 7.3.1 (Agilent Technologies, USA). The hybridization images were analyzed by GenePix Pro 6.0 (Axon Instruments, CA). KDX1 was subcloned into yeast multicopy vector ( pRS426 ) Total RNA was isolated using TRIzol reagent (Life Technologies). RNA concentrations were determined by measuring absorbance at 260nm. Labeling reactions were performed with a Bioprime labeling kit (Invitrogen) in a volume of 50 M-NM-