Comment[ArrayExpressAccession]	E-GEOD-45205
MAGE-TAB Version	1.1						
Public Release Date	2013-12-01
Investigation Title	Effects of miR-106a~363 blocking sponge on Sk-ES-1 Ewing Sarcoma Cell line Gene Expression Profile						
Comment[Submitted Name]	Effects of miR-106a~363 blocking sponge on Sk-ES-1 Ewing Sarcoma Cell line Gene Expression Profile
Experiment Description	The objective of this study was to determine the effects of miR-106a~363 blockade on the gene expression profile of Ewing Sarcoma cell lines (Sk-ES-1 cells) Microarray analysis was performed on 3 samples from Sk-ES-1 cells stably expressing the control CXCR4 miRNA sponge and 3 samples from Sk-ES-1 cells stably expressing the miR-106a~363 cluster blocking miRNA sponge						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Dylla	Jedlicka	Dylla				
Person First Name	Layne	Paul	Layne				
Person Email	Layne.Dylla@ucdenver.edu						
Person Affiliation	University of Colorado Anschutz Medical Campus						
Person Address	Pathology, University of Colorado Anschutz Medical Campus, 12800 E. 19th Ave. MS8104, Aurora, CO, USA						
Person Roles	submitter						
Protocol Name	P-GSE45205-1	P-GSE45205-5	P-GSE45205-6	P-GSE45205-2	P-GSE45205-3	P-GSE45205-4	P-GSE45205-7
Protocol Description	CEL files were processed in Partek Genomic Suite using RMA Quantile normalization; intensity values were scaled by log2 transformation ID_REF =  VALUE = RMA-normalized, log2 transformed intensity values	Fragmented ssDNA was labeled with the Affymetrix WT Terminal Labeling and Hybridization kit according to the instructions, using Affymetrix proprietary biotinylated labeling reagent.	Samples were hybridized using the Gene Atlas Microarray System (Affymetrix) hybridization chamber; 20 hours at 48 C	Sk-ES-1 cells were stably transduced with either the control CXCR4 miRNA sponge or the miR-106a~363 blocking miRNA sponge	Sk-ES-1 cells were grown in DMEM 10% FBS media under standard tissue culture practices. Cells were stably transduced with the designated miRNA sponge and maintained under 0.5ug/ml puromycin selection for 5-7 days prior to harvesting for RNA	Total RNA was harvested using the RNEasy mini kit (Qiagen). 100ng total RNA per sample was used for array analysis	Samples were scanned and imaged using the Gene Atlas Microarray System Fludics and Imaging equipment (Affymetrix)
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	CELL LINE						
Experimental Factor Type	cell line						
Comment[SecondaryAccession]	GSE45205						
Comment[GEOReleaseDate]	2013-12-01						
Comment[ArrayExpressSubmissionDate]	2013-03-15						
Comment[GEOLastUpdateDate]	2013-12-01						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-45205.sdrf.txt