Comment[ArrayExpressAccession]	E-GEOD-44793						
MAGE-TAB Version	1.1						
Public Release Date	2013-11-14						
Investigation Title	DNA-damaging agent and immune inducer synergistically induced defence gene expression in Arabidopsis						
Comment[Submitted Name]	DNA-damaging agent and immune inducer synergistically induced defence gene expression in Arabidopsis						
Comment[AEExperimentDisplayName]	Transcription profiling by array of Arabidopsis seedlings grown after DNA-damaging agent bleomycin (BLM) and/or immune inducer 2, 6-dichloroisonicotinic acid (INA) treatment to identify synergistically induced defence genes						
Experiment Description	We used microarrays to identify differentially expressed genes after DNA-damaging agent bleomycin (BLM) and/or immune inducer 2, 6-dichloroisonicotinic acid (INA) treatment. We focused on those genes that were synergistically induced by co-treatment (BLM+INA). Arabidopsis seedlings were treated with 4 μg/ml BLM and/or low INA (10 μM). There are 4 treatments: control (CK), INA, BLM and BLM+INA. Each treatments have three biological replicates. There are 12 samples in total.						
Term Source Name	ArrayExpress	EFO					
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Yan	Yan	Wang	Dong			
Person First Name	Shunping	Shunping	Wei	Xinnian			
Person Email	hillson78@gmail.com						
Person Affiliation	Duke University						
Person Address	Duke University, 127 Science Dr., Durham, North Carolina, USA						
Person Roles	submitter						
Protocol Name	P-GSE44793-1	P-GSE44793-5	P-GSE44793-6	P-GSE44793-2	P-GSE44793-3	P-GSE44793-4	P-GSE44793-7
Protocol Description	The data were normalized with RMA algorithm using Gene-Spring GX default analysis settings ID_REF =  VALUE = RMA signal intensity	RNA probes were labelled using MessageAmp Premier RNA Amplification Kit (Ambion).	Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix ATH1 GeneChip (Santa Clara, CA, U.S.A).	Seedlings were treated with 4 μg/ml BLM and/or low INA (10 μM)	Arabidopsis seedlings were grown on MS plates at 21-24�C, 16-hour day length, 60% relative humidity.	Total RNA was isolated using RNeasy Mini Kit (Qiagen)	GeneChips were scanned using the Axon Genepix Scanner (Genepix 4000B).
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	stimulus						
Experimental Factor Type	stimulus						
Publication Title	Salicylic Acid Activates DNA Damage Responses to Potentiate Plant Immunity.						
Publication Author List	Yan S, Wang W, Marqu�s J, Mohan R, Saleh A, Durrant WE, Song J, Dong X						
PubMed ID	24207055						
Publication DOI	10.1016/j.molcel.2013.09.019						
Comment[SecondaryAccession]	GSE44793						
Comment[GEOReleaseDate]	2013-11-14						
Comment[ArrayExpressSubmissionDate]	2013-03-01						
Comment[GEOLastUpdateDate]	2013-11-14						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-44793.sdrf.txt