Comment[ArrayExpressAccession]	E-GEOD-44245
MAGE-TAB Version	1.1						
Public Release Date	2013-04-01
Investigation Title	Expression data from human lymphocytes						
Comment[Submitted Name]	Expression data from human lymphocytes
Experiment Description	Baseline gene expression of human lymphocytes 24 hours after 5 Gy gamma irradiation We used microarrays to detail the global gene expression of human lymphocytes after irradiation Compare the gloable gene expression of human lymphocyte 24 hours after exposure to doses between 0 and 5 Gy γ rays						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Han	Lee	Wu	Han			
Person First Name	Leng	Won	Joseph	Leng			
Person Mid Initials		H	C				
Person Email	hanleng0926@hotmail.com						
Person Affiliation	Stanford University						
Person Address	Stanford University, 265 Campus Dr, Stanford, CA, USA						
Person Roles	submitter						
Protocol Name	P-GSE44245-1	P-GSE44245-6	P-GSE44245-5	P-GSE44245-2	P-GSE44245-3	P-GSE44245-4	P-GSE44245-7
Protocol Description	ID_REF =  VALUE = log2 RMA signal	GeneChips were scanned using theAffymetrix GeneChip Scanner 3000 7G	Hybridization were followed standard Affymetrix protocol in Affymetrix GeneChip Hybridization Oven 640	γ-Irradiation (5 Gy)	Extraction of lymphocyte total RNA from whole blood sample was performed according to the manufacturer's instructions.	Biotinylated cRNA were prepared according to the standard Affymetrix protocol	The data were analyzed with Affymetrix Expression Console using Affymetrix default analysis settings and RMA as normalization method.
Protocol Type	bioassay_data_transformation	image_aquisition	hybridization	specified_biomaterial_action	nucleic_acid_extraction	labeling	feature_extraction
Experimental Factor Name	TREATMENT						
Experimental Factor Type	treatment						
Comment[SecondaryAccession]	GSE44245						
Comment[GEOReleaseDate]	2013-04-01						
Comment[ArrayExpressSubmissionDate]	2013-02-11						
Comment[GEOLastUpdateDate]	2013-04-01						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-44245.sdrf.txt