Comment[ArrayExpressAccession]	E-GEOD-43670
MAGE-TAB Version	1.1					
Public Release Date	2013-03-07
Investigation Title	The 3’UTR of FMR1 mRNA is a target of miR101, miR129-5p and miR-221: implications for the molecular pathology of FXTAS at the synapse.					
Comment[Submitted Name]	The 3’UTR of FMR1 mRNA is a target of miR101, miR129-5p and miR-221: implications for the molecular pathology of FXTAS at the synapse.
Experiment Description	While FMR1 is silenced in Fragile X syndrome (FXS), its expression is elevated (2-8 fold) in premutated individuals. These people may develop the Fragile X-associated Tremor Ataxia Syndrome (FXTAS), a late onset neurodegenerative disorder characterized by ataxia and parkinsonism. In addition, people carrying the premutation can be affected by a set of neurological and behavioural disorders during young age. Problems of memory have been detected in these patients as well in the mice model for FXTAS. To date little is known concerning the metabolism of FMR1 mRNA, notwithstanding the importance of the finely tuned regulation of the expression of this gene. In the present study we identified three microRNAs that specifically target the 3’UTR of FMR1 and can modulate its expression throughout the brain and, in particular, at the synaptic level. The expression level of miR-221 is reduced in brain and synaptosomal preparations of young FXTAS mice suggesting a general deregulation of transcripts located at the synapse of these mice. By transcriptome analysis we show here a robust deregulation of the expression levels of genes involved in learning, memory and autistic behavior, Parkinson disease and neurodegeneration. Interestingly, many of those deregulated mRNAs are target of the same miRNAs that modulate the expression of FMR1 at the synapse. Knock-In versus Wild Type, 3 replicates.					
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl				
Person Last Name	Lebrigand	Bardoni	Barbry	Mari	Lebrigand	
Person First Name	Kevin	B	P	B	K	
Person Email	mari@ipmc.cnrs.fr					
Person Affiliation	IPMC/CNRS					
Person Phone	0493957792					
Person Address	IPMC/CNRS, 660 route des lucioles, Valbonne - Sophia-Antipolis, France					
Person Roles	submitter					
Protocol Name	P-GSE43670-1	P-GSE43670-5	P-GSE43670-4	P-GSE43670-2	P-GSE43670-3	P-GSE43670-6
Protocol Description	ID_REF =  VALUE = Log2(signal)	Arrays were scanned using the Agilent SureScan High Resolution Scanner.	Agilent : 200.0 ng at 65 degree_C during 17 hours.	Chomzynski extraction protocol.	Agilent - Low RNA input QuickAmp.	LimmaGUI analysis within custom R script with Quantile Normalization.
Protocol Type	bioassay_data_transformation	image_aquisition	hybridization	nucleic_acid_extraction	labeling	feature_extraction
Experimental Factor Name	VARIATION	G				
Experimental Factor Type	variation	g				
Publication Title	The 3' UTR of FMR1 mRNA is a target of miR-101, miR-129-5p and miR-221: implications for the molecular pathology of FXTAS at the synapse.					
Publication Author List	Zongaro S, Hukema R, D'Antoni S, Davidovic L, Barbry P, Catania MV, Willemsen R, Mari B, Bardoni B					
PubMed ID	23390134					
Publication DOI	10.1093/hmg/ddt044					
Comment[SecondaryAccession]	GSE43670					
Comment[GEOReleaseDate]	2013-03-07					
Comment[ArrayExpressSubmissionDate]	2013-01-22					
Comment[GEOLastUpdateDate]	2013-03-07					
Comment[AEExperimentType]	transcription profiling by array					
SDRF File	E-GEOD-43670.sdrf.txt