Comment[ArrayExpressAccession]	E-GEOD-43623
MAGE-TAB Version	1.1						
Public Release Date	2013-05-01
Investigation Title	Gene expression in murine stromal cells with MEK inhibition						
Comment[Submitted Name]	Gene expression in murine stromal cells with MEK inhibition
Experiment Description	comparative expression between stromal MS5 cells treated with (MS5_PD18) or without (MS5_DMSO) MEKi Stromal MS5 cells were treated during 7 days with MEKi (1µM) or diluent						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Dumont	Uzan					
Person First Name	Florent	Benjamin					
Person Email	florent.dumont@inserm.fr						
Person Affiliation	INSERM						
Person Address	GENOM'IC, INSERM, 27 rue du faubourg Saint Jacques, Paris, France						
Person Roles	submitter						
Protocol Name	P-GSE43623-1	P-GSE43623-5	P-GSE43623-6	P-GSE43623-2	P-GSE43623-3	P-GSE43623-4	P-GSE43623-7
Protocol Description	the data were RMA normalized  and  analysed with Partek Genomic Suit ID_REF =  VALUE = RMA signal	Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Following the GeneChip whole transcript (WT) Sense Target labelling assay kit Affymetrix)	Following fragmentation, 10 ug of cDNA were hybridized for 16 hr at 45C on GeneChip murine Gene from Affymetrix GeneChips were washed and stained in the Affymetrix Fluidics Station 450.	stromal cells line	cells growth in alpha-MEM medium(Gibco) supplemented FBS(10%)	Trizol extraction of total RNA was performed according to the manufacturer's instructions.	GeneChips were scanned using the GCS 3000 scanner (Affymetrix)
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	COMPOUND						
Experimental Factor Type	compound						
Comment[SecondaryAccession]	GSE43623						
Comment[GEOReleaseDate]	2013-05-01						
Comment[ArrayExpressSubmissionDate]	2013-01-18						
Comment[GEOLastUpdateDate]	2013-05-02						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-43623.sdrf.txt