Comment[ArrayExpressAccession]	E-GEOD-43452
MAGE-TAB Version	1.1							
Public Release Date	2013-01-12
Investigation Title	Gene profile of glioblastoma cells treated with y15 and temozolomide							
Comment[Submitted Name]	Gene profile of glioblastoma cells treated with y15 and temozolomide
Experiment Description	The gene expression profiles were identified in glioblastoma cells treated with FAK inhibitor Y15, temozolomide alone or with combination of Y15 and Temozolomide DBTRG and U87 were treated with FAK inhibitor Y15 at 10 microM for 24 h; U87 cells were treated with Temozolomide 100 microM for 24 h and Y15+temozolomide at the same dose as each agent alone							
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl						
Person Last Name	Hu	Golubovskaya	Cance	Liu	Hu	Conroy	Huang	Ho
Person First Name	Qiang	Vita	William	Song	Qiang	Jeffrey	Grace	Baotran
Person Email	Qiang.Hu@RoswellPark.org							
Person Affiliation	Roswell Park Cancer Institute							
Person Address	Roswell Park Cancer Institute, Elm & Carlton Streets, Buffalo, NY, USA							
Person Roles	submitter							
Protocol Name	P-GSE43452-1	P-GSE43452-5	P-GSE43452-4	P-GSE43452-2	P-GSE43452-3	P-GSE43452-6		
Protocol Description	ID_REF =  VALUE = quantile normalized and background subtracted	Following washing and staining, the BeadChips were imaged using the Illumina iScan Reader to measure fluorescence intensity at each probe.	750ng of the biotin labeled cRNA probes were mixed with hybridization reagents and hybridized overnight to the HumanHT-12 v4 BeadChips.	Total RNA from 10-20 mg fresh frozen tissues were prepared using the RNeasy mini kits (Qiagen, Valencia, CA) following manufacturer’s instructions. After elution, RNA samples were concentrated by EtOH precipitation at –20oC overnight, and resuspended in nuclease-free water. Before labeling, RNA samples were quantitated using a ND-1000 spectrophotometer (NanoDrop Wilmington, DE) and evaluated for degradation using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Samples were required to have a RIN >6.5, an OD 260:280 of 1.9-2.1, an OD 260/230 >1.5 and >1.5 28S:18S ratio of the ribosomal bands for gene expression array analysis.	Initially, 500ng total RNA was converted to cDNA, followed by an in vitro transcription step to generate biotin labeled cRNA using the Ambion Illumina Total Prep RNA Amplification Kit (Ambion, Austin, TX) as per manufacturer’s instructions.	BeadChip data files were analyzed with Illumina’s GenomeStudio gene expression module (v1.8.0)		
Protocol Type	bioassay_data_transformation	image_aquisition	hybridization	nucleic_acid_extraction	labeling	feature_extraction		
Experimental Factor Name	CELL LINE	TREATMENT						
Experimental Factor Type	cell line	treatment						
Comment[SecondaryAccession]	GSE43452							
Comment[GEOReleaseDate]	2013-01-12							
Comment[ArrayExpressSubmissionDate]	2013-01-11							
Comment[GEOLastUpdateDate]	2013-01-12							
Comment[AEExperimentType]	transcription profiling by array							
Comment[AdditionalFile:Data1]	GSE43452_non-normalized.txt							
SDRF File	E-GEOD-43452.sdrf.txt