Comment[ArrayExpressAccession] E-GEOD-43445 MAGE-TAB Version 1.1 Public Release Date 2013-01-12 Investigation Title MicroRNA profiling in primary and metastatic HCC cell lines using PLC8024 and MHCC97H derived cell lines Comment[Submitted Name] MicroRNA profiling in primary and metastatic HCC cell lines using PLC8024 and MHCC97H derived cell lines Experiment Description Goal for this study is to identified miRNA involved in metastasis development using PLC8024 and MHCC97H derived cell lines. PLC8024 derived cell lines, PLC-PT (Primary Tumor) and PLC-LM (Lung Metastasis), and MHCC97H derived cell lines, MHCC97H-PT and MHCC97H-LM were compared using the Ncode miRNA microarray platform. The experiment were repeat twice with dye-swap. Term Source Name ArrayExpress EFO Term Source File http://www.ebi.ac.uk/arrayexpress/ http://www.ebi.ac.uk/efo/efo.owl Person Last Name Yau YAU Person First Name Wing Lung Wing Person Mid Initials Simon L Person Email swlyau@hku.hk Person Affiliation The University of Hong Kong Person Phone (852)28199622 Person Fax (852)28199634 Person Address Surgery, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong, Hong Kong Person Roles submitter Protocol Name P-GSE43445-1 P-GSE43445-6 P-GSE43445-5 P-GSE43445-3 P-GSE43445-2 P-GSE43445-4 P-GSE43445-7 Protocol Description ID_REF = VALUE = Normalized Log2 Ratio (Experiment/Control) The washes were performed following the standard protocol recommended in the NCodeM-bM-^DM-" Rapid miRNA Labeling System Manual, and the arrays were scanned using a GenePixM-. 4000B microarray scanner (Molecular Devices). The two differentially labeled reactions were combined into one tube and the volume was reduced by half in a SpeedVacM-. Concentrator. BSA (50mg/ml) was then added to a total volume of 28.5ul. The samples were incubated with 28.5ul of 2X Enhanced hybridization buffer at 65oC for 10 min and then loaded onto NCodeM-bM-^DM-" Multi-Species miRNA Microarrays V2. The arrays were mounted with the Maui Mixer SL and hybridized overnight (16-20 h) at 52oC with constant mixing. Total RNA were extracted by Trizol reagent accroding to manufacturer's protocol All cell lines are maintain at 37oC incubator with 5% CO2 with DMEM supplement with 10% FBS Each total RNA sample, 1M-NM-